Antibody (Ab) responses to i.m. administered SRBC and BSA, and i.p. administered Escherichia coli lipopolysaccharide (LPS), and BW at various times after treatment, were measured in chicken lines divergently selected for high (H) and low (L) Ab responses to SRBC, and in a randombred control line (C). The Ab responses to SRBC and BSA, but not LPS, were significantly affected by line by treatment interactions. Levels of antibodies to SRBC and BSA were higher in the H line than in either the C or L line (P < 0.05). Administration of LPS did not affect Ab responses to SRBC, but Ab responses to BSA were decreased in birds that received BSA and LPS simultaneously. Body weights of C and L lines were significantly higher than BW of H line birds at all times. Lipopolysaccharide injection induced an acute, but transient reduction of BW gain, which was not affected by line. Antibody responses to SRBC and BSA were negatively correlated with BW. During the experimental period, however, percentage BW gain and humoral responsiveness were positively correlated. A higher percentage BW gain growth was seen in H line birds at the end of the experimental period. The present results confirm the hypothesized acute cachectin nature of LPS, but the relationship between live BW (gain) and immune responsiveness in chickens remains to be further clarified.
Antibody (Ab) responses to SRBC, BSA, Mycobacterium butyricum, and Escherichia coli lipopolysaccharide (LPS) were measured in two chicken lines divergently selected for high and low Ab responses to SRBC, and in a randombred control line. Levels of Ab binding SRBC, BSA, and Mycobacterium protein, but not LPS were higher in the high Ab producing (H) line than in the control (C) and low Ab producing (L) lines (P < 0.05), and at almost every time, the L line showed significantly lower titers than the H and C lines. In the H and C lines, Ab responses to SRBC were enhanced when Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA) were simultaneously administered on a separate location than SRBC. In the L line, Ab titers to SRBC and BSA were enhanced when antigen was administered emulsified in CFA. At all times until 28 d after sensitization the C and L line birds were significantly heavier than birds of the H line. Body weight, body growth, and percentage body growth were impaired in birds that received antigen emulsified in CFA, which suggested a negative relationship between BW gain and immune responses to Mycobacteria protein. Prolonged divergent selection for Ab responses to SRBC resulted into two lines that not only differ in Ab responses to T cell-dependent antigens but also in BW. In contrast to previous findings with the current lines, line differences with respect to Ab responses were not abolished by CFA treatment.
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