Breast cancer is identified as the highest cause of death in women suffering from cancer. Early diagnosis is the key to increase the survival of breast cancer victims. Molecular diagnosis using biomarkers have advanced much in the recent years. The cost involved in such diagnosis is not affordable for most of the population. The concept being investigated here is to realize a simple diagnosis system for screening cancer by way of a blood test utilizing a miRNA based biomarker with a complementary molecular beacon probe. A microfluidic platform was designed and attached with a fluorescence reader, which is portable and cost effective. Experiments were performed with 51 blood samples of which 30 were healthy and 21 were positive for breast cancer, collected against institutional human ethical clearance, IHEC 16/180-7-9-2016. miRNA 21 was chosen as the biomarker because it is overexpressed 4-fold in the serum of breast cancer patients. This work involved design of an experiment to prove the concept of miRNA over expression followed by detection of miRNA 21 using the microfluidic platform attached with a fluorescence reader and validation of the results using quantitative Real Time Polymerase Chain Reaction (qRT-PCR). The results obtained from the microfluidic device concurred with qRT-PCR results. The device is suitable for point-of-care application in a mass-screening programme. The study also has revealed that the stage of the cancer could be indicated by this test, which will be further useful for deciding a therapeutic regime.
Background: Functional mobility is defined as the ability of a person to move from place to place in the environment in order to participate in the activities of daily living. A person with overweight and obesity have a greater risk of experiencing mobility disability and those patients will have restrictions in activities at home, work, school and in the community thereby having a negative impact on their health related quality of life. Fear of falling in elderly is a major cause of loss of independence, which has an effect on the physical function in them.Purpose of the study: To find out the relationship between BMI and fear of fall on functional mobility in elderly Result: Out of 30 subjects, 15 subjects were overweight and 15 were normal BMI. Out of 15 overweight subjects, 53.3% were males and 46.7% were females. The mean age of overweight participants was 68.46±2.77. The mean scores for BMI were 27.82±1.56, for FOF 4.93±1.09and for FM 21.53±3.99. FOF and FM were negatively correlated with Overweight. The correlation was not significant. Out of 15 subjects with normal BMI, 53.3% were males and 46.7% were females. The mean age of participants with normal BMI was 68.86±3.62. The mean scores for BMI were 21.40±1.61, for FOF 5.93±.883and for FM 19.26±3.55. FOF and FM were positively correlated with normal BMI. The correlation was not significant.
Conclusion:The overweight individual have more fear of fall (FOF) compared with individuals with normal BMI. The functional mobility (FM) is decline in overweight individuals than individuals with normal BMI.Implications: A decrease in sedentary lifestyle and regular physical activity can improve health related quality of life of elderly.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.