The study aimed to evaluate the use of lactic acid as an acidifier in ration containing probiotics on blood protein profile of Sentul chicken. Sixty female Sentul chickens aged 6 months were reared for two months in a 20-unit battery cage and subjected to a completely randomized design (CRD) with four treatments and five replicates, with 3 females each for each unit. The treatments were R0 : Basal ration (BS); R1 : BS + 0.5% lactic acid; R2 : BS + 1.0% lactic acid; and R3 : BS + 1.5% lactic acid. Data were analyzed by Analysis of Variance (ANOVA) using an IBM SPSS Statistics ver. 22. The result showed that lactic acid as an acidifier in rations containing probiotics on blood protein profile of Sentul chicken did not significantly (P>0,05) affected the blood protein profile (uric acid, creatinine and blood urea nitrogen) of Sentul chicken. The average of uric acid as 5.6±1.86 mg/dl; creatinine 1.07±0.40 mg/dl and blood urea nitrogen 4.31±1.08 mg/dl. The conclusion was that the use of up to 1.5% lactic acid as an acidifier in feed created similar results in uric acid, creatinine and blood urea nitrogen. Keywords: Sentul chicken, blood protein, acidifier
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