A method is described for the preparation and purification of aflatoxin B1-1-(O-carboxymethyl) oxime from aflatoxin The overall yield was about 73–83%. The new aflatoxin B1, derivative was characterized by mass, ultraviolet, infrared, and nuclear magnetic resonance spectral analyses, and was nontoxic to 8-day-old chicken embryos when tested at a concentration of 3.48 μg/egg.
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