In the recent years, tissue culture has emerged as a promising technique to obtain genetically pure elite populations under in vitro conditions. The Cleome viscosa are used in traditional systems of medicine for the treatment of many diseases in human. The present study aims to investigate the role of assorted plant growth regulators (PGRs) on in vitro propagation and comparison of similar and dissimilar compounds of wild plant C. viscosa. Nodal explants of 1.5-2.0 cm were used to induce multiple shoots in Murashige and Skoog (MS) medium supplemented with various concentration of different plant growth regulators (PGRs) such as 6 -Benzylaminopurine (BAP), Kinetin (KIN), Naphthalene-3-acetic acid (NAA) and the bioactive constituent of wild and in vitro propagated C. viscosa plant was compared by analyzing polar and non polar extract of both the plants using Gas Chromatography -Mass Spectrometry (GC-MS) analysis. Multiple shoots were initiated within 28 days of inoculums and the various concentration of PGR had a significant role in the number of shoot formed and the in vitro regeneration of explants. The regenerated plantlets showed no morphological differences from the wild plant but the GC-MS analysis of ethanol extract showed the presence of eight compounds in wild plant and six in micropropagated while chloroform extract showed ten compounds in both plants.
Biodiversity plays a significant role in maintaining the ecosystem for their sustainable utilization. A preliminary survey was conducted to document the diversity and economic importance of the angiosperms around the Medavakkam Lake, Chengalpattu district, Tamil Nadu. The study area act as a seed bank for native species and provide suitable conditions to endemic and certain endangered species. Diversity indices of the study area was found significant, in which the species abundance, richness and evenness were found. A total of 71 species belonging to 36 families of angiosperms were documented. Among which the Dicotyledons plants were 87.32% and monocotyledon plants were 12.67% of total flora reported. The study area was dominated by the family fabaceae (14.1%) with ten species. Habit wise classification of plants reveal that trees are predominant with 32%, followed by hers (25%), Shrubs (21%), Climbers (13%), undershrubs (6%) and grasses (3%). The Simpson Diversity Index, Shannon Wiener Index and evenness index of the study area is 0.777, 1.5634 and 0.8725 respectively. These plants was found with enormous benefits to the mankind such as medicinal, edible, timber, ornamental, oil and other sources. This investigation divulges that the Medavakkam Lake has substantial angiosperm diversity served as major source for native plants having greater economic importance.
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