The functional role of the skeletal muscle intermediate filament system was investigated by measuring the magnitude of muscle force loss after cyclic eccentric contraction (EC) in normal and desmin null mouse extensor digitorum longus muscles. Isometric stress generated was significantly greater in wild-type (313 +/- 8 kPa) compared with knockout muscles (276 +/- 13 kPa) before EC (P < 0.05), but 1 h after 10 ECs, both muscle types generated identical levels of stress ( approximately 250 kPa), suggesting less injury to the knockout. Differences in injury susceptibility were not explained by the different absolute stress levels imposed on wild-type versus knockout muscles (determined by testing older muscles) or by differences in fiber length or mechanical energy absorbed. Morphometric analysis of longitudinal electron micrographs indicated that Z disks from knockout muscles were more staggered (0.36 +/- 0. 03 microm) compared with wild-type muscles (0.22 +/- 0.03 microm), which may indicate that the knockout cytoskeleton is more compliant. These data demonstrate that lack of the intermediate filament system decreases isometric stress production and that the desmin knockout muscle is less vulnerable to mechanical injury.

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The authors examined the roll-over shape alignment hypothesis, which states that prosthetic feet are aligned by matching their rollover shapes with an "ideal" shape. The "ideal" shape was considered to be the roll-over shape of the able-bodied foot-ankle system. An alignment algorithm and computational alignment system were developed to set transtibial alignments based on this hypothesis. Three prosthetic feet with considerably different roll-over shapes were either aligned using the alignment system or not aligned (i.e. used previous foot's alignment), and then were aligned by a team of prosthetists. No significant differences were found between roll-over shapes aligned by the computational alignment system and those based on standard clinical techniques (p = 0.944). Significant differences were found between the "no alignment" shapes and the prosthetist alignment shapes (p = 0.006), and between the "no alignment" shapes and the computational alignment system shapes (p = 0.024). The results of the experiment support the hypothesis that the goal of alignment is to match the prosthetic foot's roll-over shape, as closely as possible, with an "ideal" shape. The hypothesis is also supported by its ability to explain the results of previous studies. Using an "ideal" roll-over shape

The Shape&Roll Prosthetic Foot (patent pending) is an artificial foot designed for use in low-income countries, and may also be useful in industrialised nations. Its design is based on the theory that the roll-over shape of a prosthetic foot should mimic that of the non-disabled physiological foot-ankle complex during walking. This article presents the S&R foot including the unique features incorporated into its design. The results of mechanical tests indicate that the roll-over shape of the foot closely mimics the roll-over shape of the non-disabled ankle-foot complex and an expensive 'high-performance' prosthetic foot. The fatigue testing process for the S&R foot is also described. The foot has been shown to be durable according to the International Organization for Standardization (ISO) standards, with more than five samples tested to date. The Shape&Roll Foot is low in cost, simple to fabricate, light in weight, low in profile, and is highly functional for walking in respect of roll-over characteristics.

Eleven kinds of prosthetic feet that were designed for use in low-income countries were mechanically characterised in this study. Masses of the different kinds of prosthetic feet varied substantially. Dynamic properties, including damping ratios and resonant frequencies, were obtained from step unloading tests of the feet while interacting with masses comparable to the human body. Data showed that for walking, the feet can be appropriately modeled using their quasistatic properties since natural frequencies were high compared to walking frequencies and since damping ratios were small. Roll-over shapes, the effective rocker (cam) geometries that the feet deform to under walking loads, were determined using a quasistatic loading technique and a spatial transformation of the ground reaction force's centre of pressure. The roll-over shapes for most of the prosthetic feet studied were similar to the roll-over shape of the SACH (solid-ankle cushioned heel) prosthetic foot. All roll-over shapes showed a lack of forefoot support, which may cause a "drop-off" experience at the end of single limb stance and shorter step lengths of the contralateral limb. The roll-over shapes of prosthetic feet appear useful in characterization of foot function.

A field test was performed in El Salvador to evaluate the usefulness of the Shape&Roll prosthetic foot, a foot developed for low-income countries, involving 12 participants. Quantitative gait parameters were measured with a Direct Ultrasound Ranging System (DURS). Qualitative information was obtained from questionnaires administered before and after a three-week trial. The results indicate that the Shape&Roll foot widened the speed range of all participants. According to the questionnaires, the Shape&Roll foot eases walking, enabling participants to walk significantly longer distances. The participants rated its roll-over as very natural and smooth, resulting in a self-perceived reduced walking effort. Handling inclined and uneven surfaces was also rated superior to their current Solid Ankle Cushioned Heel (SACH)-like feet.

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