The present study was described thin layer chromatography (TLC) and ultra-high performance liquid chromatography (UHPLC) method for the detection of antibacterial substances in poultry muscle (breast and thigh), kidney, and liver.Materials and Methods: TLC method was used for screening detection of tetracycline, amoxicillin, ciprofloxacin, and enrofloxacin residues in poultry tissues. The samples were extracted with trichloroacetic acid (30%), diethyl ether, followed by detection in pre-coated TLC paper on ultraviolet detector. The UHPLC method was used for the quantification of antimicrobial residues in poultry tissues. Results:The residues of tetracycline were 48% in livers, 24% in kidneys, 20% in thigh muscles, and 24% in breast muscles. Ciprofloxacin residues were found 44% in liver, 42% in kidneys, 34% in thigh muscles and 30% in breast muscles. Enrofloxacin residues were found 40% in liver, 34% in kidneys, 22% in thigh muscles, and 18% in breast muscles. Amoxicillin residues were found 42% in liver, 30% in kidneys, 26% in thigh muscles and 22% in breast muscles. Most of the cases highest residues were found in liver such as tetracycline (48%), ciprofloxacin (44%), enrofloxacin (40%) and amoxicillin (42%) and almost lowest in breast muscles. In addition, nine positive samples from broiler were selected for amoxicillin residue quantification by UHPLC. It was observed that the concentration of amoxicillin residue in liver was ranging from 16.92 μg/kg to 152.62 μg/kg and in breast muscle was 45.38 μg/kg to 60.55 μg/kg, respectively. The maximum and minimum peak time was 4.7-5.2 min. Among the poultry tissues, liver had the highest level of antibiotic residues in comparison to other samples but the variation was not significant (p>0.05). Conclusions:Evidence suggests that more judicious use of antimicrobials in food animals will reduce the selection of resistant bacteria and help to preserve these valuable drugs for both human and veterinary medicine. The method described in this study is a simple, easy inexpensive which can be readily adopted by any laboratory for the detection of antibiotic residues in tissues of food-producing animals.

The study was conducted to identify the socio-economic status of the poultry farmers and economic analysis of poultry farming at Gazipur, Bangladesh. A Total of 40 poultry farms (20 broilers and 20 layers) were selected. The farmers and farming were evaluated through the analysis of data that were collected in a pre-structured questionnaire. This study revealed that comparatively rich farmers (42.5% had 4.94 acre of land) were involved in farming. There were significant variations (p<0.05) in source of investment (57.5% own, 32.5% bank loan, 7.5% from money lenders with interest and 5% from money lenders without interest), training (72.5% not received and 27.5% received), farming as an occupation for the farmers (55% main and 45% subsidiary occupation), amount of loan taken (50% have loan and 50% have no loan), level of educational knowledge (62.5% poor, 25% medium and 23.5 % high), sources of drinking water (87.5% have own tubewell), latrine condition (67.5% have sanitary latrine) and health status of the farmers (30% good, 50% medium and 20% poor). There were no significant variation (p>0.05) in farm size and management skills of the farmers. The average number of children per farmer attends to primary school, high school, college and university were 0.7, 0.75, 0.1 and 0.01 in case of boys and 0.5, 0.45, 0.07 and 0.01 in case of girls, respectively. Per bird average annual rearing cost, return, and cost benefit ratio were 925.5 BDT., 1080 BDT. and 1:1.17 in broiler and 1332.5 BDT., 2210 BDT. and 1:1.66 in layer, respectively. This study recommends although initial investment is higher; layer farming is more profitable than broiler.

The present study was carried out to know the present status including general information, feeding, breeding, housing, milking etc. and costs and returns of small dairy farms, to compare the productive and reproductive performance of crossbred and indigenous cows and to make recommendation for development of small scales dairy farming. With this view, the empirical data were collected by using pretested questionnaire. The study was conducted at preselected 30 dairy farms in Chittagong suburban area, and four months-long survey was diminished on thirty small dairy owners. It appeared that 57% farm owners belong to business class and remaining 43% to different categories. Fifty three percent took dairying as a side-business whereas only 47% took it as a main business enterprise. Major percentage of farm owner education level was Higher Secondary (60%). The dry period, calving interval, services per conception and day's open of crossbred was 98.5 419 and indigenous was 140 428 1.95 137 respectively. The study showed that there were significant (P<0.01) differences within the dry period, services per conception, calving to first service, highest and lowest milk production and lactation period of crossbred and indigenous dairy cows. The study also showed non-significant differences within calving interval for crossbred and indigenous. In case of small dairy farming, the farms were facing a lot of problems such as scarcity of feeds and fodder, high price of concentrate and lack of technical knowledge. This type of dairy farming will be a potential sector for economic development of Bangladesh.