This paper summarizes results achieved in labelling texaphyrin with radionuclides (e.g., with 177 Lu) and characterization of the metal-texaphyrin complexes. Thin layer chromatography (TLC) method was used for the quality control of the 177 Lu-labeled texaphyrin. Thermal analysis, mass spectroscopy (MS) and Fourier transform infrared spectroscopy (FT-IR) were used for the characterization of the Ca-texaphyrin, Lu-texaphyrin and Y-texaphyrin. The chemical and physical properties depend on the metal centrally placed into the cavity of the texaphyrin molecule. The labelling yield of the 177 Lu-texaphyrin by the neutron irradiated Lu-Tex complex in the LVR-15 reactor was determined to be 40 %. The TLC technique is suitable for the separation of the 177 Lu-Tex complex from the radionuclide 177 Lu. The fact that texaphyrin exhibits a high affinity of bonding to cancer cells is of great advantage as no antibody has to be applied.
This paper summarizes results achieved in labelling texaphyrin with radionuclides (e.g., with 177 Lu) and characterization of the metal-texaphyrin complexes. Thin layer chromatography (TLC) method was used for the quality control of the 177 Lu-labeled texaphyrin. Thermal analysis, mass spectroscopy (MS) and Fourier transform infrared spectroscopy (FT-IR) were used for the characterization of the Ca-texaphyrin, Lu-texaphyrin and Y-texaphyrin. The chemical and physical properties depend on the metal centrally placed into the cavity of the texaphyrin molecule. The labelling yield of the 177 Lu-texaphyrin by the neutron irradiated Lu-Tex complex in the LVR-15 reactor was determined to be 40 %. The TLC technique is suitable for the separation of the 177 Lu-Tex complex from the radionuclide 177 Lu. The fact that texaphyrin exhibits a high affinity of bonding to cancer cells is of great advantage as no antibody has to be applied.of Ca (calcium) ion by the radionuclide 177 Lu and/or 90 Y can be shown as an example. The second approach involves the production of the radionuclide labelled texaphyrin by the neutron irradiation in nuclear reactor. An alternative promising method is the synthesis of the texaphyrin conjugate. In this case, the radionuclide is bound via the bifunctional chelator (i.e. ligand) and the ligand is connected to the texaphyrin.
ExperimentalFourier transform infrared spectroscopy (FT-IR), mass spectroscopy (MS) and thermal analysis were used for characterization of metal-texaphyrin complexes (e.g., Ca-Tex, Y-Tex and Lu-Tex). Thin layer chromatography (TLC) was applied for the separation of the 177 Lu-texaphyrin complex from the radionuclide 177 Lu.Metal-texaphyrin complexes (e.g., Ca-Tex, Y-Tex, Lu-Tex) (Fig. 1) were prepared at the Department of the Analytical Chemistry, Institute of Chemical Technology in Prague. The purity of these metal-texaphyrin complexes was higher than 90 %.The Ca-texaphyrin (Ca-Tex) was dissolved in chloroform and/or methanol and then FT-IR spectra were recorded. Ca-texaphyrin solution was measured by the FT-IR spectrometer Nicolet Nexus 670 with the single-reflex ATR adapter of the MIRacle. The ZnSe M. Reková, V. Král and V. Jedináková-Křížová
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