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SummaryIn contrast to the accumulated data on nuclear transport mechanisms of macromolecules, little is known concerning the regulated release of nuclear-exported complexes and their subsequent trans-cytoplasmic movement. The bipartite begomovirus nuclear shuttle protein (NSP) facilitates the nuclear export of viral DNA and cooperates with the movement protein (MP) to transport viral DNA across the plant cell wall. Here, we identified a cellular NSP-interacting GTPase (NIG) with biochemical properties consistent with a nucleocytoplasmic transport role. We show that NIG is a cytosolic GTP-binding protein that accumulates around the nuclear envelope and possesses intrinsic GTPase activity. NIG interacts with NSP in vitro and in vivo (under transient expression), and redirects the viral protein from the nucleus to the cytoplasm. We propose that NIG acts as a positive contributor to geminivirus infection by modulating NSP nucleocytoplasmic shuttling and hence facilitating MP-NSP interaction in the cortical cytoplasm. In support of this, overexpression of NIG in Arabidopsis enhances susceptibility to geminivirus infection. In addition to highlighting the relevance of NIG as a cellular co-factor for NSP function, our findings also have implications for general nucleocytoplasmic trafficking of cellular macromolecules.

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The present work aimed to evaluate the behavior of ten fertility attributes of soil organic matter physical fractions and total organic carbon upon addition of three EM Bokashis to a Rhodic Ferralsol (FRr) and a Dystric Cambisol (CMd). An experiment was carried out in greenhouse in which the soils were placed into plastic trays and cultivated with tomato. A completely randomized design was used with four repetitions and factorial scheme of 2 × 3 + 2, consisting of two soils (FRr and CMd), three EM Bokashis (Poultry Manure Bokashi (BPM); CNPH Bokashi (BC); and Cattle Manure Bokashi (BCM)), and two controls (both soils without addition of Bokashi). The following fertility attributes were evaluated: pH, Ca 2+ , Mg 2+ , K + , Na + , P, SB, H + Al, CEC, and . Particulate organic carbon (POC) and mineral-associated organic carbon (MOC) and total organic carbon (TOC) were also investigated. Finally, the Principal Component Analysis was conducted in order to identify possible patterns related to soils when fertilized with EM Bokashi. The addition of EM Bokashi increased the soil fertility and contents of POC. Different EM Bokashi presents distinguished effects on each soil. The PCA suggests that BPM presents higher capacity to modify the analyzed chemical attributes.

Resumo -O objetivo deste trabalho foi avaliar os teores de carbono orgânico total (COT) e das frações granulométricas da matéria orgânica de um Latossolo Vermelho cultivado com hortaliças, sob diferentes sistemas de manejo e de cultivo de plantas de cobertura. O experimento foi realizado por seis anos, em delineamento de blocos ao caso, em arranjo fatorial 3x2, com três sistemas de manejo do solo -plantio direto (SPD), preparo reduzido (PPR) e preparo convencional (SPC) -e dois cultivos de cobertura, um de milho solteiro e outro de milho consorciado com mucuna-cinza (Stizolobium niveum) (MM), com quatro repetições. Os teores de COT, estimados para a camada 0,00-0,30 m, foram maiores em SPD e PPR. Independentemente da profundidade avaliada, o consórcio MM promoveu os maiores teores de carbono orgânico particulado (COP), que foram fortemente influenciados pela incorporação da palhada. Em todas as camadas, observaramse correlações negativas entre os teores de COP e os de carbono orgânico associado a minerais (COAM), indício de ocorrência do efeito "priming". Os teores de COT correlacionaram-se positivamente aos de COAM, o que destaca a importância de mecanismos de estabilização da matéria orgânica no incremento dos teores de COT no solo.Termos para indexação: carbono associado a minerais, carbono orgânico particulado, carbono orgânico total, efeito "priming", plantio direto de hortaliças, sequestro de carbono. Organic carbon pools in an Oxisol cultivated with vegetables under different managementsAbstract -The objective of this work was to evaluate the contents of total organic carbon (COT) and of the organic matter fractions in an Oxisol cultivated with vegetables under different soil management systems and with different cover crops. The experiment was carried out for six years in a randomized block design, in a 3x2 factorial arrangement with three soil management systems -no-tillage (SPD), minimum tillage (PPR), and conventional system (SPC) -, and two cover crops, one of maize and the other of maize intercropped with Stizolobium niveum (MM), with four replicates. COT levels, estimated for 0.00-0.30-m, soil depth were higher in SPD and PPR. Regardless of the evaluated soil depth, MM intercropped system promoted higher contents of particulate organic carbon (COP), which were strongly affected by straw incorporation. In all soil depths, negative correlations were observed between the contents of COP and those of carbon associated to minerals (COAM), which indicate the occurrence of priming effect. COT contents were positively correlated to those of COAM, stressing the importance of soil organic matter stabilization mechanisms for improving COT contents in the soil.Index terms: carbon associated to minerals, particulate organic carbon, total organic carbon, priming effect, no-tillage for vegetables, carbon sequestration. IntroduçãoPara que ocorra sequestro de carbono, os sistemas de manejo devem adicionar elevadas quantidades de biomassa ao solo, causar o mínimo distúrbio em sua estrutura, além de promover a conser...

Several agro-industrial, livestock, and food wastes can be recycled to create biofertilizers. This diversity of raw materials can result in nutritional imbalance and an increase in heavy metal content, which could make the final product unfeasible. Thus, the chemical characterization of the raw materials and their influence on the sustainable and safe production of biofertilizers need to be better understood. In this context, the objective of the present study was to evaluate the chemical characteristics of agro-industrial residues used in the manufacture of an aerobic liquid biofertilizer. We analyzed the macronutrient, micronutrient, and trace metal contents of seven waste products used as raw materials to create a biofertilizer. In addition, a survey of secondary biofertilizer data from different residues was carried out that showed great heterogeneity in the chemical compositions of these residues, which has a direct impact on the agronomic efficiency of these biofertilizers. The characterization revealed that some materials may be contaminants of the soil, due to high levels of trace metals, especially cadmium. We conclude that the generation of detailed inventories, such as those of the nutrient and heavy metal contents of the raw materials and biofertilizers produced, is indispensable for the correct recommendation of biologically-based inputs in agriculture.

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