A type A Clostridium perfringens enterotoxin was partially purified by ammonium sulfate precipitation (0 to 15%) and was submitted to polyacrylamide gel electrophoresis (7%). A specific enterotoxin antiserum was obtained by inoculating a rabbit with the polyacrylamide gel strip containing the enterotoxin. This serum gave only one precipitin line with purified enterotoxin and cellular extract in immunodiffusion and immunoelectrophoresis. The titer (1:8) in counter-immunoelectrophoresis was sufficient to detect 0.39 ~tg/ml enterotoxin by this technique. This serum neutralized the mouse lethality, cytotoxicity and plating efficiency of Vero cells.
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