1. Hyperpolarizing potentials evoked by electrical stimulation of the optic tract were studied in projection cells of the rat dorsal lateral geniculate nucleus (LGN) in vitro. In the same cells the effects of gamma-amino butyric acid (GABA), baclofen and acetylcholine (ACh) were also investigated. 2. In the majority of cells a short- (SHP) (34 ms) and a long-lasting (LHP) (240 ms) hyperpolarizing potential could be recorded in the presence and in the absence of a preceding EPSP. They were blocked by tetrodotoxin (1 microM) and were more sensitive than the monosynaptic EPSP to a low-Ca2+-high-Mg2+ solution. 3. The SHP was associated with a marked decrease (75%) in input resistance, was blocked by bicuculline (1-100 microM) and its reversal potential (-67 mV) was dependent on the extracellular Cl- concentration. 4. The LHP was associated with a smaller decrease (45%) in input resistance and its reversal potential (-76 mV) was dependent on the extracellular K+ concentration. It was increased by bicuculline (100% at 50 microM) and nipecotic acid (30% at 10 microM), blocked by Ba2+ (1 mM), and unaffected by eserine (1-10 microM), neostigmine (1-10 microM) or by recording with EGTA-filled electrodes. In the presence of bicuculline, a single LHP was able to evoke, as a rebound response, a low-threshold Ca2+ spike that was, however, not followed by another LHP (or any other long-lasting hyperpolarization). 5. Ionophoretic applications of GABA evoked in the same cell a Cl- -dependent hyperpolarization (reversal potential: -65 mV) and/or depolarization, both of which were associated with a marked decrease (91%) in input resistance and abolished by bicuculline. GABA was also able to evoke a bicuculline-insensitive, K+-dependent hyperpolarization that had a reversal potential of -75 mV and was associated with a smaller decrease (43%) in input resistance. 6. Baclofen, applied by ionophoresis, pressure ejection or in the perfusion medium (1-100 microM), produced a hyperpolarization that had a reversal potential of -79 mV and was associated with a decrease (45%) in input resistance. 7. In the majority of cells (thirty-seven out of forty) ACh evoked a slow depolarization and only in three cells a hyperpolarization which had a reversal potential of -80 mV.(ABSTRACT TRUNCATED AT 400 WORDS)
SUMMARY1. The membrane properties and the electrotonic structure of neurones in the ventral and dorsal lateral geniculate nucleus (l.g.n.) of the rat were studied using an in vitro slice preparation.2. Following electrophysiological characterization, horseradish peroxidase (HRP) was injected intrasomatically and the morphological features of impaled cells were characteristic of principal neurones of the rat ventral and dorsal l.g.n.3. Neurones in the ventral l.g.n. had a higher input resistance but similar membrane time constants (X0) and resting potentials than cells in the dorsal l.g.n.4. Using a simple neuronal model, the electrotonic length (L) and the dendritic to somatic conductance ratio (p) were calculated and found to be similar for cells in both divisions of the l.g.n. The mean value of L (0 7) and p (1P5) suggest that both groups of neurones are electrotonically compact.5. The width and after-hyperpolarization of directly evoked action potentials, but not their threshold or their amplitude, were different between cells of the ventral and dorsal l.g.n.6. At potentials more negative than -55 mV, a slow rising and falling potential could be evoked in each neurone (n = 310) of the dorsal l.g.n. but only in three cells of the ventral l.g.n. (n = 94). The electrophysiological and pharmacological properties of this potential were identical with those of the low-threshold Ca2+-dependent potential observed in other thalamic nuclei.7. These results indicate that some of the passive and active membrane properties of ventral and dorsal l.g.n. neurones are different. The implications of these findings for the control of the integrative capability and the response of 1.g.n. neurones to visual stimulation are discussed.
SUMMARY1. The electrophysiological and pharmacological properties of the excitatory post-synaptic potentials (e.p.s.p.) evoked by electrical stimulation of the optic tract were studied in projection neurones of the ventral and dorsal lateral geniculate nucleus (l.g.n.) of the rat in vitro.2. No difference was found in the rise time of e.p.s.p.s. recorded in the dorsal and ventral l.g.n. and in their threshold for action potentials. At membrane potentials more negative than -60 mV, e.p.s.p.s. in the dorsal l.g.n. were always followed by a Ca2+-dependent potential. Its amplitude could easily reach threshold for generating an action potential and thus evoke firing from an e.p.s.p. that was subthreshold at resting potential. No Ca2+ potential was observed to follow e.p.s.p.s. recorded in the ventral l.g.n.3. At resting potential the excitability of dorsal and ventral cells was unaffected following an initial shock to the optic tract. However, in dorsal neurones, at potentials more negative than -60 mV, the presence of Ca2+ potentials evoked by the e.p.s.p.s. resulted in a period of decreased excitability.4. Using intrasomatic injection of Cs+ the reversal potential (E) of the e.p.s.p. and of the depolarization produced by glutamate could be measured in the same l.g.n.neurone. They were: Eepsp, -0 9 mV; and Eglut-3.9 mV.5. y-D-glutamylglycine (DGG), an excitatory amino acid antagonist, reversibly inhibited the e.p.s.p. and depolarization produced by quisqualate and glutamate by a competitive action. The concentration of DGG that produced 50 % inhibition (IC50) was 2-7 mM.6. D-2-amino-5-phosphonovalerate (APV), the potent and selective N-methyl-D-aspartate (NMDA) antagonist, had no effect on the e.p.s.p. both in the presence and absence of Mg2+. The isomers of 2-amino-4-phosphonobutyrate (APB) were inactive or had a non-specific action on the e.p.s.p.
The development of membrane properties, firing patterns, and ␦ oscillations in neurons of the cat dorsal lateral geniculate nucleus (dLGN) was investigated in vitro during the first 7 postnatal weeks. Compared with adult neurons, the resting membrane potential was more depolarized at postnatal days 1-9 (P1-P9), the input resistance was higher at P1-P7, and action potentials had a higher threshold and a smaller amplitude at P1-P3 and a longer duration at P1-P9. At P1-P3 trains longer than 200 msec were rarely observed, and trains with more than three action potentials were only present in 41% of the neurons, whereas at P1-P7 the normalized slope of the instantaneous frequencies at the first five interspike intervals was smaller than in the adult. A long-lasting (up to 6 sec) afterhyperpolarization followed a short train of action potentials in 88 and 30% of neurons at P1-P3 and P30-P32, respectively, but it was rarely observed in the adult. The low-threshold Ca 2ϩ potential could evoke a burst of action potentials since P1.However, at P1-P7 the number of action potentials per burst was smaller (range, one to five), and at P1-P9 their maximum instantaneous frequency was lower (Ͻ190 Hz) than in the adult (range, six to eight, and 344 Hz, respectively). No ␦ oscillations were observed until P17, and their frequency (0.36 Hz) was lower than that in the adult (1.8 Hz). The percentage of neurons displaying ␦ oscillations and their frequency reached adult values by the end of the seventh postnatal week, i.e., well after the maturation of the membrane properties and firing patterns (second postnatal week). In conclusion, the maturation of the electrophysiological properties of thalamocortical neurons in the cat dLGN is completed later than the retinogeniculate axon segregation (Shatz CJ, 1983), and the immaturity of the oscillatory, and not of the burst-firing, activity is a limiting factor in the development of ␦ waves (Jouvet-Mounier et al., 1970).
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