Widespread occurrence of ZnO nanoparticles in environment follows the growing number of applications either in technology or agriculture. The impact of five forms of nanoparticulate ZnO on copper, manganese and iron uptake by Pisum sativum L. cultivated in Hoagland solutions was investigated. Plants were collected after twelve days of zinc administration. Effect of bulk ZnO has also been studied. Initial zinc concentration was 100 mg L −1. Nanoparticles were characterized by the transmission electron Microscopy, Dynamic Light Scattering and Zeta potential measurements. Metal contents were analyzed using the Atomic Absorption Spectrometry with flame atomization for samples digested in a microwave closed system. Analysis of variance indicated that zinc species at either molecular or nanoscale levels altered Cu, Mn and Fe uptake and their further transport in pea plants. In particular, significant reduction of Mn and Fe combined with the Cu increase was observed. Additive interactions originated by nanoparticles affect the heavy metals uptake and indicate pollutants migration pathways in plants. Unfortunately, regulations for the plant cultivation were formulated when anthropogenic nanoparticles were not in common use. they underestimate complexity of metals interactions in either plant or habitat. Our results indicate that these additive interactions cannot be neglected and deserve further investigations. Green pea (Pisum sativum L.) is one of the most extensively cultivated grain legumes worldwide. Plants are well adapted to diverse soil zones in either cool or mild climatic regions 1. Their seeds are rich in proteins, carbohydrates, dietary fibers, vitamins as well as minerals and are commonly used as vegetable or important protein source. The latter is of particular relevance when animal feed is concerned 2. The global production of pea is steadily growing as indicated by the projected Compound Annual Growth Rate (CAGR) of 5.9% and has approached 20 million tonnes in 2018 3. Pea genetics was thoroughly studied and in combination with better plant breeding methods has led to variety of improved plant species 4. Nowadays, pea is an important non model plant widely used in applied system biology studies 5. The pea genome has not been completely determined as yet. Nevertheless, it is being frequently applied as a model plant with the almost complete transcript coverage 6. The impact of nanoparticles (NPs) on plants physiology and their nutritional quality is usually assessed using two leading methodologies as presented by Jośko and Oleszczuk 7. Initially, the long-term growth in soils supplemented with representative concentrations of investigated nanoparticles was applied 8,9. Nowadays, the soilless plant cultivation is gaining increasing popularity 10. Hydroponic techniques promote plant growth in nutrient solutions. Their usage by far exceeds the laboratory scale and they have found numerous applications in commercial crop production 11. Several advantages of hydroponic cultures are highly appreciated, i.e. the s...
Cerium oxide nanoparticles (CeO2 NPs) and zinc oxide nanoparticles (ZnO NPs) are emerging pollutants that are likely to occur in the contemporary environment. So far, their combined effects on terrestrial plants have not been thoroughly investigated. Obviously, this subject is a challenge for modern ecotoxicology. In this study, Pisum sativum L. plants were exposed to either CeO2 NPs or ZnO NPs alone, or mixtures of these nano-oxides (at two concentrations: 100 and 200 mg/L). The plants were cultivated in hydroponic system for twelve days. The combined effect of NPs was proved by 1D ANOVA augmented by Tukey’s post hoc test at p = 0.95. It affected all major plant growth and photosynthesis parameters. Additionally, HR-CS AAS and ICP-OES were used to determine concentrations of Cu, Mn, Fe, Mg, Ca, K, Zn, and Ce in roots and shoots. Treatment of the pea plants with the NPs, either alone or in combination affected the homeostasis of these metals in the plants. CeO2 NPs stimulated the photosynthesis rate, while ZnO NPs prompted stomatal and biochemical limitations. In the mixed ZnO and CeO2 treatments, the latter effects were decreased by CeO2 NPs. These results indicate that free radicals scavenging properties of CeO2 NPs mitigate the toxicity symptoms induced in the plants by ZnO NPs.
Cerium dioxide nanoparticles are pollutants of emerging concern. They are rarely immobilized in the environment. This study extends our work on Pisum sativum L. as a model plant, cultivated worldwide, and is well suited for investigating additive interactions induced by nanoceria. Hydroponic cultivation, which prompts accurate plant growth control and three levels of CeO2 supplementation, were applied, namely, 100, 200, and 500 mg (Ce)/L. Phytotoxicity was estimated by fresh weights and photosynthesis parameters. Additionally, Ce, Cu, Zn, Mn, Fe, Ca, and Mg contents were analyzed by high-resolution continuum source atomic absorption and inductively coupled plasma optical emission techniques. Analysis of variance has proved that CeO2 nanoparticles affected metals uptake. In the roots, it decreased for Cu, Zn, Mn, Fe, and Mg, while a reversed process was observed for Ca. The latter is absorbed more intensively, but translocation to above-ground parts is hampered. At the same time, nanoparticulate CeO2 reduced Cu, Zn, Mn, Fe, and Ca accumulation in pea shoots. The lowest Ce concentration boosted the photosynthesis rate, while the remaining treatments did not induce significant changes. Plant growth stimulation was observed only for the 100 mg/L. To our knowledge, this is the first study that demonstrates the effect of nanoceria on photosynthesis-related parameters in peas.
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