Context: In vitro propagation or tissue culture of plants offers a rapid means of producing large quality of clonal planting stock and propagation that are difficult to establish conventionally. Biotechnological tools are important for multiplication and genetic enhancement of the plants by adopting techniques such as in vitro regeneration and genetic transformations.Objective: Effect of different plant growth regulators (PGRs) and their concentration on multiple shoot regeneration and callus formation was studied in Indigofera viscosa.Materials and Methods: In vitro plant regeneration was achieved in nodal and shoot tip explants. The explants were cultured on MS medium supplemented with BAP and NAA.Results: The nodal explants exhibited a greater number of healthy multiple shoots. The maximum callus induction was observed on MS medium supplemented with 1.5 mg L-1 2,4-D. NAA and BAP combination proved to be the most effective treatment for promoting shoot multiplication. IBA was found to be the best rooting hormone than IAA or NAA. The plantlets showed high survival rate in the soil.Conclusion: The present investigation clearly established and demonstrated the method of obtaining the new plantlets in Indigofera viscosa supported by different hormone concentrationsJ. bio-sci. 22: 53-58, 2014
Climbers are currently understood to have a range of important ecological functions in forest dynamics. Climbers are already recognized as an important group for tropical biodiversity, playing a key role in ecosystem level processes and providing resources for pollinators and dispersers. The present study is an attempt to document different climber species and their uses in Point Calimere Wildlife and Birds Sanctuary, Tamil Nadu, India. The present study recorded 53 herbaceous climbers and 21 lianas from all the forests types of Point Calimere Sanctuary, covering 25 families. Considering all climbers and lianas, 40 species are stem twiners, 2 species are branch twiners, 4 are spiny Climbers, 19 species are tendril climbers and 8 species are hook climbers. Most of the lianas are distributed in scrub forests and many climbers are recorded in wet lands. 53 medicinal climbers are recorded in the study area. Roots and leaves are widely used to treat diseases. To obtain a better comprehension of the floristic, ecological and biogeographical patterns of climbing species at a global scale, it is of the utmost importance that future studies include an increased number of subtropical and temperate sites.
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