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Case studies are necessary to assess the effects of changes to tree species on the physicochemical and chemical properties of soils. To achieve this, the fine earth under five tree species was investigated. This study was performed in the Breuil-Chenue experimental forest site located in the Morvan Mountains (France). This site contains two adjacent blocks with replicated stands. The native forest (old beech and oak coppice with standards) was partially clear-felled and replaced in 1976 with mono-specific plantations of European beech, Norway spruce, Laricio pine and Douglas fir. The same changes in soil properties were revealed in both blocks, thus confirming the tree species effect. The percentage of exchangeable acidity on the cation exchange capacity (CEC) was greater under spruce, Douglas fir and pine than under the other species. Spruce stands, and to a lesser extent those of Douglas fir and pine, had a less acidic soil pH than hardwood stands (which was unusual in view of the data in the literature) and smaller CEC values. The small quantities of carbon added to the soil under these tree species provide an explanation for these effects through a partial control of both CEC and pH. This case study thus demonstrated that the tree species effect was not unequivocal and different criteria are necessary for its interpretation. Tree species significantly influenced certain aspects of the chemical properties of topsoil and have the potential to have an impact on current soil fertility.

The impacts of plant species on the microbial communities and physico-chemical characteristics of soil are well documented for many herbs, grasses and legumes but much less so for tree species. Here, we investigate by rRNA and ITS amplicon sequencing the diversity of microorganisms from the three domains of life (Archaea, Bacteria and Eukaryota:Fungi) in soil samples taken from the forest experimental site of Breuil-Chenue (France). We discovered significant differences in the abundance, composition and structure of the microbial communities associated with two phylogenetically distant tree species of the same age, deciduous European beech (Fagus sylvatica) and coniferous Norway spruce (Picea abies Karst), planted in the same soil. Our results suggest a significant effect of tree species on soil microbiota though in different ways for each of the three microbial groups. Fungal and archaeal community structures and compositions are mainly determined according to tree species, whereas bacterial communities differ to a great degree between rhizosphere and bulk soils, regardless of the tree species. These results were confirmed by quantitative PCR, which revealed significant enrichment of specific bacterial genera, such as Burkholderia and Collimonas, known for their ability to weather minerals within the tree root vicinity.

The mineralogy of bulk and rhizosphere soils was compared to assess the effect of roots on mineral weathering in a Typic Dystrochrept supporting Norway spruce (Picea abies [L.] Karst) and oak (Quercus sessilifl ora Smith). In an experimental forest site (Breuil-Chenue, France), systematic soil sampling was performed in forty pits. The soil adhering to the roots was considered as rhizosphere soil. The remaining material was regarded as bulk soil. The mineralogy of the clay-sized particles of both fractions was determined by x-ray diffraction (XRD). Amorphous solid phases were estimated by extracting Fe and Al with hot Na-citrate and dithionite-citrate-bicarbonate. Total chemical analyzes were performed on the clay-sized particles via BaCl 2 -saturation and an iso-zirconium weathering balance was calculated. The XRD demonstrated an increase of illite-like minerals in the rhizosphere of both species and the selective extractions revealed a decrease in the amorphous phases. The total chemical analyzes showed that the rhizosphere clay-sized fraction contained signifi cantly more Si and K (for oak only) and less Fe and Al than the bulk soil. By way of the iso-zirconium weathering balance, these losses of Fe and Al in the rhizosphere were estimated at several tens of kilograms by hectare for the surface horizon (0-3 cm). This study demonstrates that, despite the short duration of the contact between the active part of a root and the solid mineral phase, the intensity of the processes occurring in the rhizosphere signifi cantly increases mineral weathering.

Minerals constitute an ecological niche poorly investigated in the soil, in spite of their important role in biogeochemical cycles and plant nutrition.To evaluate the impact of minerals on the structure of the soil bacterial communities, we compared the bacterial diversity on mineral surfaces and in the surrounding soil. Three pure and calibrated minerals (apatite, plagioclase and a mix of phlogopite-quartz) were buried into the organo-mineral layer of a forest soil. After a 4-year incubation in soil conditions, mineral weathering and microbial colonization were evaluated. Apatite and plagioclase were the only two significantly weathered minerals. The analysis of the 16S rRNA gene sequences generated by the cloning-sequencing procedure revealed that bacterial diversity was higher in the surrounding soil and on the unweathered phlogopitequartz samples compared with the other minerals. Moreover, a multivariate analysis based on the relative abundance of the main taxonomic groups in each compartments of origin demonstrated that the bacterial communities from the bulk soil differed from that colonizing the minerals. A significant correlation was obtained between the dissolution rate of the minerals and the relative abundance of Beta-proteobacteria detected. Notably, many sequences coming from bacteria colonizing the mineral surfaces, whatever the mineral, harbored high similarity with efficient mineral weathering bacteria belonging to Burkholderia and Collimonas genera, previously isolated on the same experimental site. Taken together, the present results provide new highlights concerning the bacterial communities colonizing minerals surfaces in the soil and suggests that the minerals create true ecological niches: the mineralosphere.

Although minerals represent important soil constituents, their impact on the diversity and structure of soil microbial communities remains poorly documented. In this study, pure mineral particles with various chemistries (i.e., obsidian, apatite, and calcite) were considered. Each mineral type was conditioned in mesh bags and incubated in soil below different tree stands (beech, coppice with standards, and Corsican pine) for 2.5 years to determine the relative impacts of mineralogy and mineral weatherability on the taxonomic and functional diversities of mineral-associated bacterial communities. After this incubation period, the minerals and the surrounding bulk soil were collected to determine mass loss and to perform soil analyses, enzymatic assays, and cultivation-dependent and -independent analyses. Notably, our 16S rRNA gene pyrosequencing analyses revealed that after the 2.5-year incubation period, the mineral-associated bacterial communities strongly differed from those of the surrounding bulk soil for all tree stands considered. When focusing only on minerals, our analyses showed that the bacterial communities associated with calcite, the less recalcitrant mineral type, significantly differed from those that colonized obsidian and apatite minerals. The cultivation-dependent analysis revealed significantly higher abundances of effective mineral-weathering bacteria on the most recalcitrant minerals (i.e., apatite and obsidian). Together, our data showed an enrichment of Betaproteobacteria and effective mineral-weathering bacteria related to the Burkholderia and Collimonas genera on the minerals, suggesting a key role for these taxa in mineral weathering and nutrient cycling in nutrient-poor forest ecosystems.IMPORTANCE Forests are usually developed on nutrient-poor and rocky soils, while nutrient-rich soils have been dedicated to agriculture. In this context, nutrient recycling and nutrient access are key processes in such environments. Deciphering how soil mineralogy influences the diversity, structure, and function of soil bacterial communities in relation to the soil conditions is crucial to better understanding the relative role of the soil bacterial communities in nutrient cycling and plant nutrition in nutrient-poor environments. The present study determined in detail the diversity and structure of bacterial communities associated with different mineral types incubated for 2.5 years in the soil under different tree species using cultivationdependent and -independent analyses. Our data showed an enrichment of specific bacterial taxa on the minerals, specifically on the most weathered minerals, suggesting that they play key roles in mineral weathering and nutrient cycling in nutrientpoor forest ecosystems.KEYWORDS mineral chemistry, bacterial communities, forest soil, mesh bags, mineral weatherability

It has been rarely questioned as to whether the enrichment of specific bacterial taxa found in the rhizosphere of a given plant species changes with different soil types under field conditions and under similar climatic conditions. Understanding tree microbiome interactions is essential because, in contrast to annual plants, tree species require decades to grow and strongly depend on the nutritive resources of the soil. In this context, we tested using a natural toposequence the hypothesis that beech trees select specific taxa and functions in their rhizosphere based on the soil conditions and their nutritive requirements. Our 16S rRNA gene pyrosequencing analyses revealed that the soil type determines the taxa colonizing the beech rhizosphere. A rhizosphere effect was observed in each soil type, but a stronger effect was observed in the nutrient-poor soils. Although the communities varied significantly across the toposequence, we identified a core beech rhizosphere microbiome. Functionally, GeoChip analyses showed a functional redundancy across the toposequence, with genes related to nutrient cycling and to the bacterial immune system being significantly enriched in the rhizosphere. Altogether, the data suggest that, regardless of the soil conditions, trees enrich variable bacterial communities to maintain the functions necessary for their nutrition.