Three years of full-scale operation of the CaptorR process had been completed at the Moundsville/Glen Dale Wastewater Treatment Plant (WWTP) treating a design flow of 8706 m3/d (2.3 mgd). The CaptorR process applied a combination of attached and suspended growth processes by adding polyurethane foam media to activated sludge process, raising the equivalent MLSS concentration. The CaptorR system was designed as a separate front portion of the activated sludge process, with one third of the total HRT of the activated sludge process. The suspended MLSS in the Captor basin was around 900-1700 mg/L and the equivalent media based attached MLSS was maintained at 6,500-12,000 mg/L. It has been found that soluble CBOD5 removal, nitrification and partial denitrification occurred simultaneously in the Captor basin. The influent loadings to the Captor chamber were at 100-130 mg/L of soluble BOD5, 20-30 mg/L NH3-N, and 50-70 mg/L of TSS at flow rates of 5678-10031 m3/d (1.5-2.65 mgd). Over the last three years, 95% soluble CBOD5 removal, 70-90% nitrification and 40-60% denitrification were achieved at an HRT of 50-90 minutes in the Captor chamber. The plant effluent characteristics consistently met 2-4 mg/L of CBOD5, 1-4 mg/L of TSS, and 2-4 mg/L of Ammonia at low temperatures of 12°C-15°C. The HRTs were 2.5-5 hours for secondary aeration with a sludge age of 3-5 days for the activated sludge portion.
A technique was developed to measure the equivalent MLSS in the fixed film Captor® media-activated sludge process. The technique accounts for the wear in the media as well as unequal wear in different media. The microbial population variations encountered during three years of full scale operation of the Captor® process, at the Moundsville WWTP were recorded. It has been found that fixed film media seem to support predominantly rotifers, followed by free swimming ciliates, and single stalk ciliates. In contrast, the suspended growth activated sludge population contained lower level organisms, mostly free swimming ciliates, followed by stalk ciliates, flagellates, and a few rotifers.
The dried mango fruit processing industrial waste (MIW) used as carbon source for the production of pectinase from fungal strains. Eight fungal strains were isolated from MIW and screened for their ability to produce pectinase by pectin clear zone (PCZ) technique. Fusarium sp. (PSTF1) showed highest PCZ value of 52 mm. The physico-chemical characteristics of the medium were standardized for high production of pectinase. The highest production of pectinase in submerged fermentation observed at temperature—28 °C, pH-6.0, inoculum-size 0.6/25 ml, incubation—72 h, substrate concentration—0.6 g/100 ml, carbon source-fructose (1 %). The effect of different amino acids, vitamins also observed. Under these optimal conditions the highest activity 81.9718 µmol/ml of pectinase was observed. The Fusarium sp. (PSTF1) has been considered as the best pectinase producer in submerged fermentation of MIW. The cheap waste raw material used as best carbon source for high production of high value pectinase.
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