It has been argued that dairy cows with a high genetic milk production potential can maintain high milk production even with total omission of the dry period. Further, when omitting the dry period, cows are believed to experience fewer metabolic changes during the transition from late gestation to early lactation compared with cows having a traditional dry period. The performance and metabolic response to omission of the dry period for cows with an expected peak milk yield higher than 45 kg/d were studied in 28 Holstein dairy cows. The cows were followed in late gestation and in the subsequent 5 wk of early lactation. Fourteen cows were milked through late gestation (CM) and another 14 dairy cows underwent a 7-wk dry period (DRY). In the early lactation period, the cows had the same dry matter (DM) intake but cows in the CM group had a 22% reduction in milk yield compared with the cows in the DRY group. At calving, the experimental groups had the same average body weight and body condition score and there were no significant differences in body weight and body condition score changes in early lactation. However, the cows in the CM group compared with the cows in the DRY group had a higher plasma concentration of glucose and insulin and a lower plasma concentration of nonesterified fatty acids and beta-hydroxybutyrate in the following 5 wk of early lactation. Furthermore, the cows in the CM group had lower liver triacylglycerol concentration and higher liver glycogen concentration in the following early lactation. It is concluded that, even in dairy cows with an expected peak milk yield above 45 kg/d, omission of the dry period results in a relatively high reduction in milk yield in the following early lactation. Furthermore, these cows are in less metabolic imbalance in the following early lactation.

The objective was to examine the effects of insulin under euglycaemic conditions on liver long chain fatty acids (LCFA) metabolism with special focus on the aetiology of hepatic lipidosis in early lactation. A 4-day hyperinsulinaemic-euglycaemic clamp (clamp) was conducted on four dairy cows starting in weeks 4 and 17 postpartum. Insulin was infused continuously (1 microg/kg BW per h) and a 50% glucose solution was infused to maintain euglycaemia. Liver biopsies were taken 6 days before, the last day of, and 5 days after the clamp, and blood samples were taken in the same period. In the liver tissue, the relative triglyceride content decreased (P < 0.01) and the glycogen content increased (P < 0.0001) in response to the clamp. Hepatic in vitro palmitate oxidation capacity was lowest during the clamp period and could be explained by a significant decrease in incomplete oxidation (ketogenesis) (P < 0.04) and a tendency to a decreased complete oxidation of palmitate (P < 0.10). Plasma non-esterified fatty acids concentration was decreased during the clamp in early lactation (P < 0.05) but there was no effect on the mid-lactation clamp. The present study shows that increased insulin under euglycaemic conditions seems to depress hepatic LCFA oxidation capacity. However, in terms of preventing hepatic lipidosis, the anti-lipolytic effect of insulin on adipose tissue, which results in decreased mobilization of and hence hepatic load with LCFA, appears more important.

The objective of this study was to examine the effects of diet energy density (high versus low) and increased milk yield, induced by increased milking frequency (two versus three times daily), on the hepatic status of triacylglycerol (TAG) and glycogen content and hepatic long chain fatty acid (LCFA) oxidation capacity in early lactation in a 2 x 2 factorial design. Forty multiparous Danish-Holstein dairy cows were used from 8 weeks before to 8 weeks after calving. Liver biopsies and blood samples were taken in weeks -2, 2, and 7 from calving. The cows fed the high energy density diet, compared with the cows fed the low energy density diet, had an 18 and 28% higher milk production and net energy intake, respectively. Milk yield was increased by 10% when the cows were milked three times compared with twice daily. Complete (CO2 production) and incomplete (ketone body production) LCFA oxidation capacity in the liver were 35 and 32% higher, respectively, and liver TAG content was 48% lower for the cows fed the high energy density diet compared with the low energy density diet. Overall there was no effect of milking frequency on liver parameters. However, a significant interaction between diet and milking frequency showed that the cows milked three times daily and fed the low energy density diet had the lowest liver LCFA oxidation (CO2 and ketone body) capacity. Furthermore, these cows had the numerically highest liver TAG content. The results for liver LCFA metabolism are discussed in relation to the plasma concentration of metabolites and insulin. In conclusion, cows in early lactation given a high energy density diet will, in general, have a lower risk of high TAG infiltration in the liver.

We studied the influence of short-term changes in water intake in 4 lactating Holstein cows on diurnal fluctuation of packed cell volume (PCV), freezing point of blood (FPblood), freezing point of milk (FPmilk), and the relationship between changes in FPblood and FPmilk. The experiment lasted 108 h and was divided into 3 periods: 1) control (38 h); 2) dehydration/rehydration with 4 consecutive 12-h sequences: 8 h without water, 0.5-h access to water, 1.5 h without water, and 2-h access to water; and (3) 22 h for reconstitution. Cows were milked at 12-h intervals. Blood was sampled from the jugular vein hourly throughout the experiment, and at 0, 15, 30, 60, 90, 120, 150, 180, 210, and 240 min after initiated rehydration following the 8-h dehydration sequences. Intakes of free water and water in feed were recorded every hour. The PCV was negatively affected by water intake within the hour before sampling. Dehydration lowered FPblood steadily, whereas FPblood increased by 0.024 degrees C within 30 min following a large water intake in the rehydration period. The FPblood was not significantly influenced by actual water intake, but was highly correlated with the available water pool at time of blood sampling. The FPmilk correlated positively with the FPblood collected 1 h before milk sampling, indicating a delay in the transfer of water from plasma to milk. In summary, FPblood and FPmilk decrease during dehydration and increase during rehydration. Rehydration following a long dehydration period caused an increase in FPmilk within 1 h, but not above the initial level for FPmilk of the cow.

The purpose of this experiment was to investigate how early lactating cows adjust their metabolism and production to acute, but moderate changes in the energy density of the diet. Sixty dairy cows were randomly assigned to one of four treatments: two change-over groups (HNH and NHN) and two control groups (HHH and NNN), where H and N refer to a high and normal energy density in the total mixed ration (TMR), respectively. The experimental period covered the first 9 weeks post calving, which was split up in three 3-week periods. Thus, cows assigned to HNH or NHN shifted TMR in weeks 4 and 7 after calving while cows assigned to HHH or NNN were fed the same TMR for all 9 weeks. Results from cows on treatment HNH were compared with group HHH while cows on treatment NHN were compared with group NNN. When the diet changed from N to H and H to N, cows increased and decreased their dry-matter intake (DMI), respectively compared with control groups. Cows adjusted milk yield accordingly to changes in DMI, although not always significantly. Energy-corrected milk yield was not significantly affected by any of the changes in the energy density of the diet but generally showed same tendencies as milk yield. Non-esterified fatty acids (NEFA), beta-hydroxybutyrate in blood and milk and triacylglycerol and glycogen content in the liver were not significantly affected by changes in the energy density of the diet, except from NEFA at one change. Glucose increased more when the diet changed from N to H and increased less when the diet changed from H to N, compared with control groups, although not always significantly. Collectively, these results suggest that cows adjust their DMI and partly milk yield according to the energy density of the diet and therefore only limited effects were observed in physiological parameters.