Biofilms act as physical barriers to the immune system and drugs used by the host, resulting in antimicrobial resistance. Biofilms reduce the chances of eradicating infections and can result in relapses and backsliding after conventional treatment. Biofilms have a big impact on food safety in the food industry; many foodborne outbreaks have been linked to pathogenic bacteria that can form a biofilm. Biofilm-associated infections can cause not only severe symptoms but also serious side effects and even death. The findings of an experimental study of pathogenic bacteria like Pseudomonas aeruginosa, Salmonella enteritidis, and Staphylococcus aureus forming biofilms are presented in this article. The process of biofilm formation and its development phases were displayed with preserved architectonics using light and scanning electron microscopes. The amount of biofilm formed was influenced by the growth medium as well as the incubation conditions and time. Biofilm-forming microbes are a common cause of complicated and recurrent diseases, and they're usually linked to MDR bacteria, which account for nearly 80% of all refractory nosocomial infections. Medical device- and tissue-associated biofilm infections are two types of biofilm infections. Understanding the pathogenesis and factors that contribute to biofilm formation, as well as the disruption and dispersal mechanisms of biofilms, will aid in the development of improved anti-biofilm strategies. Overall, this literature review can serve as a single source of information about microbial biofilm formation and mitigation strategies, which could be extremely useful to biofilm researchers. Keywords: biofilm; pathogenic bacteria; infection; anti-biofilm.
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