Nanofertilizers have become a pioneer approach in agriculture research nowadays. In this paper we investigate the delivery of chitosan nanoparticles loaded with nitrogen, phosphorus and potassium (NPK) for wheat plants by foliar uptake. Chiotsan-NPK nanoparticles were easily applied to leaf surfaces and entered the stomata via gas uptake, avoiding direct interaction with soil systems. The uptake and translocation of nanoparticles inside wheat plants was investigated by transmission electron microscopy. The results revealed that nano particles were taken up and transported through phloem tissues. Treatment of wheat plants grown on sandy soil with nano chitosan-NPK fertilizer induced significant increases in harvest index, crop index and mobilization index of the determined wheat yield variables, as compared with control yield variables of wheat plants treated with normal non-fertilized and normal fertilized NPK. The life cycle of the nano-fertilized wheat plants was shorter than normal-fertilized wheat plants with the ratio of 23.5% (130 days compared with 170 days for yield production from date of sowing). Thus, accelerating plant growth and productivity by application of nanofertilizers can open new perspectives in agricultural practice. However, the response of plants to nanofertilizers varies with the type of plant species, their growth stages and nature of nanomaterials.

The adverse effects of either NaCl or mannitol on growth, nitrogen content, and antioxidant system in Vicia faba seedlings were investigated. The role of exogenous ascorbic acid in increasing resistance to these stressors was also evaluated. Thus, with an increase in concentration of either NaCl or mannitol in culture media, a progressively greater significant decrease in percentage germination, in growth parameters, and in nitrogen constituents of the germinating beans, was observed. On the other hand, amide-, nitrate-, and total soluble-N contents appeared to show a progressive significant increase. Exogenous addition of ascorbic acid (4 mM) to the stressful media induced a pronounced significantly increased percentage germination and the growth attributes, whereas nitrogen constituents were variably changed in relation to values maintained in beans treated with either NaCl or mannitol. Furthermore, exogenous addition of ascorbic acid to NaCl or mannitol media induced a significant increase in the contents of ascorbate and glutathione and enzymatic antioxidant activities, in particular, in beans treated with the three highest concentrations of NaCl or mannitol, throughout the period of the experiments (12 days). Thus, ascorbic acid ameliorates the adverse effects of the stressful media; the magnitude of amelioration being a function of the type and the concentration of the stressful agent as well as of the duration of treatment. The importance of the above-mentioned changes in growth and metabolism to stress tolerance in broad bean is discussed.

The use of chitosan (CS) as a carrier for slow fertilizer release is a novel trend. The potential effect of this system in agriculture is still debatable. Here, chitosan (CS) nanoparticles were obtained by polymerizing methacrylic acid (PMAA) for the entrapment of nitrogen, phosphorous and potassium (NPK) nanoparticles (NP), each at a time to form CS-PMAA-NPK NPs complex. The impact of this complex was evaluated using garden pea ( var. Master B) plants. Five-day-old pea seedlings were treated through their root system with CS-PMAA-NPK NPs at concentrations of 1, 0.5, 0.25, 0.125 and 0.0625 of the stock solution () for 1, 2, 4 and 7 days. In general, CS-PMAA-NPK NP complex reduced root elongation rate and resulted in the accumulation of starch at the root tip in a dose-dependent manner within the treated plants. Interestingly, the lowest concentrations of 0.0625 and 0.125 had induced mitotic cell division (MI = 22.45 ± 2.68 and 19.72 ± 3.48, respectively) compared with the control (MI = 9.09 ± 3.28). In addition, some of major proteins such as convicilin, vicilin and legumin β were upregulated in plants treated with these low concentrations too. However, all concentrations used exhibited genotoxic effect on DNA based on the comet assay data after 48 h of treatment. Thus, it is highly recommended to consider the negative effects of this carrier system on plants and environment that may arise due to its accumulation in the agricultural fields.

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Prasad and Zeeshan 10 in Plectonema boryanum and Agarwal 11 in Cassia seedlings. The UV enhancement of CAT and peroxidase (POD) activities, which are both responsible for detoxification of H 2 O 2 are probably equally important in the detoxification of H 2 O 2 , generated by SOD in Cassia seedlings. Increases in activities of peroxidases by UV radiation have been observed in several species including Cassia species 12 and potato.

He b a M a h m o u d Mo h a m m a d A b d e l -A zi zM o h a m m e d N a g i b A b d e l -Gh a n y H a s a n e e n A ya Mo h e b O me r Foliar application of nano chitosan NPK fertilizer improves the yield of w heat plants grow n on tw o different soils ABSTRACT: Foliar application of nano chitosan nitrogen, phosphorus and potassium (NPK) fertilizer decreased the life cycle of wheat plants with the ratio of 23.5% (130 days for yield production from date of sowing). Treatment of wheat plants with nano chitosan NPK fertilizer induced signif icant increases in all yield parameters, as compared with control yield parameters of normal non -fertilized and normal fertilized NPK wheat plants. Transmission electron microscopy investigations showed that nanoparticles were present in phloem tissues whi ch mean that nanoparticles were taken up and transported through phloem route from leaves to stem down to roots. Clay-sandy soil showed better results than those obtained for clay soil. Foliar application of nano fertilizer to wheat plants proved to be important to improve yield parameters of wheat plants and shorten the life cycle of the crop. KEY WORDS:Nanofertilizers, Clay soil, Clay -sandy Soil, Uptake, W heat. CORRESPONDENCE:He

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