Pengembangan tepung komposit di negara-negara tropis terutama diarahkan pada pengembangan produk tepung bebas-gluten berbahan baku lokal. Penelitian bertujuan untuk mendapatkan formulasi optimal tepung komposit berbahan baku tepung onggok fermentasi (TOF), tapioka dan gum xanthan (GX) untuk menghasilkan tepung komposit dengan karakteristik optimal sebagai tepung bebas-gluten. Optimasi karakteristik tepung komposit dilakukan menggunakan metode respon permukaan dengan model Central Composite Design. Hasil optimasi menggunakan metode numerik menunjukkan bahwa karakteristik optimal produk tepung komposit diperoleh pada formulasi 1) TOF: 82,46%, tapioka: 17,54%, GX: 0,10%, 2) TOF: 83,85%, tapioka: 16,15%, GX: 0,10%, dan 3) TOF: 84,13%, tapioka: 15,87%, GX: 0,10%. Formulasi-formulasi optimal selanjutnya dianalisis karakteristik pastingnya, mencakup  viskositas maksimum, viskositas awal pendinginan, viskositas akhir pendinginan, viskositas retrogradasi, dan viskositas balik. Pengujian karakteristik pasting pada formulasi optimal menunjukkan bahwa formulasi TOF: 82,46%, tapioka: 17,54%, GX: 0,10% karena memiliki karakteristik pasting terbaik dengan viskositas maksimum  215 BU, viskositas awal pendinginan 166 BU, viskositas akhir pendinginan 217 BU, viskositas retrogradasi 208 BU, dan viskositas balik 49 BU. Oleh karena itu, penelitian ini berhasil menyimpulkan bahwa tepung komposit dengan formulasi TOF: 82,64%, tapioka: 17,54%, GX: 0,10% merupakan perlakuan terbaik yang dapat dijadikan sebagai alternatif tepung bebas-gluten berbahan baku lokal. Hal ini menunjukkan bahwa tepung komposit dengan formulasi 82,64% tepung onggok terfermentasi, mempunyai potensi khusus untuk dikembangkan sebagai tepung bebas-gluten berbahan baku lokal.Optimization of Characteristic of Fermented Cassava Bagasse Flour-Based Composite Flour using Response Surface MethodologyAbstractThe development of composite flour in tropical countries is mainly directed at gluten-free flour made from local raw materials. The research was aimed to obtain an optimal formula of composite flour that was made of fermented cassava bagasse flour (FCBF), tapioca, and xanthan gum (XG) to produce composite flour with best characteristics quality as gluten-free flour. Optimization of the composite flour characteristics was done using the response surface methodology with the central composite design model. Optimization results using numerical methods shown that the optimal characteristics of composite flour were obtained in formulas 1) FCBF: 82.46%, tapioca: 17.54%, XG: 0.10%, 2) FCBF: 83.85%, tapioca: 16.15%, XG: 0.10%, and 3) FCBF: 84.13%, tapioca: 15.87%, XG: 0.10%. The optimal formulas were analyzed for pasting characteristics, including peak temperature, maximum viscosity, trough viscosity, final viscosity, retrogradation viscosity, and setback viscosity. The results of pasting characteristic test on optimal formulation showed that FCBF formulation: 82.64%, tapioca: 17.50%, XG: 0.10% had the best pasting properties with peak viscosity of 215 BU, trough viscosity of 166 BU, final viscosity of 217 BU, retrogradation viscosity of 208 BU, and setback viscosity of 49 BU. As conclusion, formula of FCBF: 82.64%, tapioca: 17.50%, XG: 0.10% was chosen as the best formula to produce gluten-free flour from local raw materials. This indicates that composite flour with formulation of 82,64% fermented cassava bagasse flour, potentially developed as gluten-free flour made from local raw materials.

Saccharomyces cerevisiae is the most commonly used yeast in the fermentation process on high starch/carbohydrate substrates, for example, cassava pulp, a by-product from the tapioca industry. In addition to the pure culture of S. cerevisiae, the fermentation process of cassava pulp can be done using tape yeast (a consortium of yeast, fungal and bacteria). The objective of this research was to study the growth kinetics of S. cerevisiae and tape yeast on cassava pulp fermentation. The growth kinetics of Saccharomyces cerevisiae and tape yeast was observed through cells number, rate of starch degradation, rate of dietary fiber degradation, rate of cyanide degradation, and rate of protein formation. The research showed that the S. cerevisiae pure culture could grow better during cassava pulp fermentation compared to tape yeast, which is reflected by the logarithmic growth rate (up to 72 h versus 48 h). The difference in the growth rate between S. cerevisiae pure culture and tape yeast will cause a difference in starch degradation rate (73.02 mg/h versus 65.09 mg/h), dietary fiber degradation rate (87.33 mg/h versus 21.09 mg/h), cyanide degradation rate (92.57.10−2 ppm/h versus 97.49.10−2 ppm/h), protein formation rate (48.92 mg/h versus 50.08 mg/h).

Limbah hasil pertanian merupakan sumber serat yang sangat besar. Limbah hasil pertanian banyak terdapat di Provinsi Lampung antara lain adalah jerami padi dan jagung. Pemanfaatan limbah pertanian merupakan salah satu solusi untuk mendapatkan material penghasil selulosa. Nanoselulosa merupakan salah satu pemanfaatan dari selulosa yang sangat bermanfaat bagi kehidupan. Nanoselulosa dapat dibagi menjadi 3 tipe utama yaitu selulosa nanokristal, selulosa nanofibril, dan nanoselulosa bakterial. Nanoselulosa memiliki diameter 1-100 nm dan panjang 500-2000 nm. Beberapa teknik dikembangkan untuk mengekstrak nanoselulosa dari selulosa diantaranya hidrolisis asam, hidrolisis enzimatis, dan proses mekanis. Adapun penggunaan masing-masing metode ekstraksi dimungkinkan menghasilkan tipe dan properti nanoselulosa yang berbeda. Penelitian ini bertujuan untuk melakukan sintesis nanoselulosa dari limbah hasil pertanian dengan berbagai konsentrasi asam untuk menghasilkan selulosa dengan ukuran nano terbaik berkisar antara 1 – 100 nm. Variasi konsentrasi H2SO4 yang digunakan yaitu 45%, 55%, dan 65%. Dari penelitian ini menghasilkan serbuk selulosa dengan ukuran nano yang bervariasi. Ukuran nanoselulosa yang dihasilkan berkisar antara 356,5 nm – 764,2 nm untuk limbah jerami padi, dan 422,6 nm – 634,0 nm untuk limbah kulit jagung

Abstract. Resistant starch is one important component determining the characteristics of a functional food. The aim of the research was to determine the cooling time optimum in the autoclaving-cooling treatment to increase the resistance starch content corn-based rice analogues, with 6 level of cooling time (0 hours/control, 12 hours, 24 hours, 36 hours, 48 hours and 60 hours). The results showed that cooling at 4 0 C for 60 hours would increase the resistant starch content (6.27% to 15.38%), dietary fiber content (14.53% to 20.17%); and decrease the digestible starch content (61.81% to 52.70%). Cooling time level at 4°C for 24 hours, would increase the sensory score of corn-based rice analogues then back down until cooling time level of 60 hours. Microscopic analysis of granular structure using SEM indicated that cooling time had a linear correlation with cracks intensity on the granule surface of the corn-based rice analogues. The high content of resistant starch showed that the application of cooling time level at 4°C for 24 hours would improve the functional properties of corn-based rice analogues with sensory characteristics remain favorable to panelists.

Resistant starch is a starch fraction that can not be hydrolyzed by digestive enzymes in the small intestine and classified as a prebiotic compound. Increasing the content of resistant starch to a certain amount in Siger Rice (modified tiwul) will decrease the cooking quality. The objective of this research was to study the relationship between resistant starch content and cooking quality of Siger Rice (eating quality, texture, and taste). The increase of resistant starch content in siger rice was done by the application of autoclaving-cooling cycling treatment, through steam stages, cooling to room temperature, followed by cooling at 4°C for 0 hours/control, 12 hours, 24 hours, 36 hours and 48 hours. The results showed that the increase of resistant starch content ≤ 10% (9.85%) will improve the quality characteristics of Siger Rice for all organoleptic scores, i.e., eating quality (7,15 to 8,2), texture (7.05 to 8.35), and flavor (6.95 to 8.15); on the contrary, the increase of resistant starch content more than 10% (14.25%) will decrease the cooking quality characteristics of Siger Rice for all organoleptic scores, i.e., eating quality (7.15 to 6.8), texture (7.05 to 6.6), and taste (6.95 to 6.4).