Abstract. A begomovirus was detected by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in the weed Mimosa invisa, collected from vegetable-and fruit-growing areas of the Malaysia Peninsula. The infected plants displayed yellowing and stunting symptoms. Sequencing of the 1.3-kb PCR amplicon showed that this virus has greatest sequence similarity (92%) to Ageratum yellow vein china virus, a white-fly transmitted begomovirus not yet identified in Malaysia. This is the first report of a begomovirus infection in the potential weedy reservoir, M. invisa.Mimosa invisa is a common weed found in most vegetablegrowing areas in Malaysia. During a survey of whiteflytransmitted geminiviruses, M. invisa plants showing yellowing and stunting symptoms (Fig. 1a) were collected from two farms growing either papaya and/or vegetables. Figure 1b shows an uninfected M. invisa plant. Although M. invisa plants occupied less than 5% of the total crop growing areas in the two farms they could potentially act as virus reservoirs.The begomovirus positive diagnosis on two plant samples from each location was confirmed by triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) using antibodies specific to Tomato yellow leaf curl virus (TYLCV, Adgen, United Kingdom). Polymerase chain reaction (PCR), using degenerate primers, was then used to further characterise the virus. This approach has been shown to be very useful for uncovering potentially new infections of begomoviruses. DNA from the M. invisa leaves from the same samples as above was extracted using a DNeasy Plant Mini Kit (Qiagen, UK) according to the manufacturer's instructions. PCR was performed using a begomovirus-specific degenerate primer pair PAV1v722 and PAC1c1960, which is predicted to anneal within the coat protein (CP) open reading frame and the AC1 open reading frame encoding the replicon associated proteins, respectively (Muniyappa et al. 2000). All samples produced viral DNA amplicons of~1.3 kb, which incorporated part of the CP and AC1 open reading frames and the entire open reading frames of proteins AC2 and AC3. Viral DNA amplicons were cloned into a pGEMT easy vector system II (Promega, USA) according to the manufacturer's instructions and sequenced. Nucleotide sequences of the DNA amplicons were compared with all published nucleotide sequences from the GenBank database using BLAST analysis (Altschul et al. 1997).Comparison of the 1.3-kb cloned sequences from each virus isolate, showed a 92% nucleotide sequence identity with that of
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