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The response of broiler chickens to 2 levels of endo-1,4-beta xylanase and endo-1,3-beta glucanase combination (with and without), 3 levels of digestible Lys (0.8, 0.9, and 1.0%), and 2 levels of canola meal (CM; 20 and 30%) were evaluated in 2 x 3 x 2 factorial arrangement. A total of 2,448 male Hubbard broiler chicks were fed on practical mash diets having 2,750 kcal of ME.kg(-1) and 19.6% CP from 1 to 42 d of age. The BW gain was significantly reduced when 30% CM was added in the diets during 1 to 21 d. Feed:gain and mortality were also observed to be high. No significant effect of enzyme addition or Lys level was observed on feed intake, BW gain, feed:gain, and mortality during the starter phase. When the data were pooled for 42 d, BW gain and feed:gain were unaffected by enzyme addition or Lys levels. A depression in breast weight was observed due to 30% CM or 0.8 and 0.9% digestible Lys at 43 d. Leg weights were significantly depressed by enzyme addition or increasing digestible Lys to 1.0% of the diets. The AME, nitrogen digestibility, and antibody titers against Newcastle and infectious bursal diseases were also unaffected by the dietary treatments. In conclusion, the 30% CM is not recommended in broiler diets especially during starter phase (1 to 21 d). However, the CM may be used up to 30% of the diets during finishing phase. The digestible Lys can be lowered to 0.8% when amino acids in proportion to digestible Lys follow the ideal AA ratio. The glucanase and xylanase cocktail have no pronounced effect on the growth performance, nutrient digestibility, and carcass characteristics.

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