Background: Hepatocellular Carcinoma (HCC) is the leading cause of cancer deaths worldwide as well as in Egypt. We aimed to use Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) gene editing technique to induce forced down-regulation of the circRNA which consequently modified miRNA expression in HepG2 cell line to prove the regulatory relationship between the RNA parts of an in silicodetected competing endogenous RNA network in HCC
A B S T R A C TThe aim of the present study was to evaluate the changes in skeletal muscle which may occur after in ovo injection of Myo-Pro gene . A total number of 200 Fayoumi fertilized pure breed eggs were obtained from EL-Tkamoly poultry project -EL-Azab -Fayoum and divided into two equal groups. Control group, n= 100 that was injected with saline, and treated group n = 100 was injected with MyoPro gene. To check both possibilities, we examined the gastrocnemius muscle fibers number and diameter (cross sectional area). The results revealed that Chicken hatched from Myo-Pro injected eggs showed a significant increased muscle masses. This increase in muscle mass and subsequently meat production is due to hypertrophy rather than hyperplasia. From the obtained results, using Myo-Pro gene causing improvement in skeletal muscle fiber (diameter and number) in Fayoumi chicken.
The current study aimed at evaluation the effects of in ovo injection of the myostatin inhibitor, myostatin pro-peptide (MyoPro) gene on growth performance of Fayoumi chicken. A total number of 200 Fayoumi fertilized pure breed eggs were obtained from EL-Tkamoly poultry project -EL-Azab -Fayoum. Eggs were divided into two equal groups. Group 1(control group, n=100) was injected with saline, and group 2 (treated group, n=100) injected with Myo-pro gene. Growth parameters were recorded (body weight, body weight gain, feed consumption per group was measured for calculating feed conversion ratio). The obtained results revealed that injection of myo-pro to Fayoumi eggs resulted in highly significant increase in body weight and weight gain during all experimental period when compared with control group. Feed conversion ratio of Myo-pro injected group was significantly lower than control group. However, food intake showed no significant change between the two groups. From the obtained results, injection of Myo-Pro gene causing marked increase in body weight gain and improvement in feed conversion ratio without change in food intake in fayoumi chicken.
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