FMD virus type A/1/ Egypt 2006 was inactivated with 0.1 M of BEI (Binary ethylene imine) formed by cyclization of 2-Bromoethyl-amine hydrobromide (BEA) in 0.2 N NaoH at 37 o C with PH 8.0 for 24 hours. The virus was complete inactivated after 15 hours post inactivation. No residual virus particles were detected when inoculated in tissue culture. The inactivation rates are linear with a regular loss of titer ranged from 0.5-1.0 log 10 / hour. Control sample of virus at 37 o C without BEI showed only a loss of 1.0 log from the original infectivity titer after 24 hours. The sample of virus which kept at -20 o C, without BEI, showed loss 0.3 log 10 from its original infectivity titer after 24 hours. There is no change in the complement fixing antigen before and after inactivation process with BEI inactivator and in the CFT ⅛ dilution of antigen was stable (fixed) pre and post inactivation of virus. Also it was found that the inactivation rate of BEI was higher than the inactivation with pure Ethylenimine (EI) and formalin.
Foot and mouth disease virus (FMDV) is the most economically important veterinary pathogen due to its highly infectious nature and ability to cause persistent infection.In the period betweenAugust to October2014, an outbreak ofFoot and Mouth Disease(FMD) occurred in Egypt. This study was designated to isolate and detect the current strains of FMDV circulating in Egypt. Twenty sixtissue samples were collected from clinically diseased cattle and buffaloes from Kafrelsheikh province.The isolation of the causative agent wasdone by inoculation of baby mice intraperitoneally then passed to Baby Hamster Kidney-21 (BHK-21) cells. The inoculated mice showed paralysis and death, while the infected BHK-21 showed clear CPE (cell rounding and cell death). Molecular characterizations of the FMDV were doneusing universal primers followed by serotype specific primers for A, O, C and SAT2. Only serotypes SAT2 and A were detected with an overall detection rate of 53.8%.Results of phylogenetic tree of the current FMDV strains revealed that there is no significant divergence among the recent isolated strains and other Egyptian isolated strains from different localities.
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