The potential of resveratrol to mimic beneficial effects of calorie restriction (CR) was investigated. We compared the effects of both CR (70% of ad libitum energy intake) or resveratrol (2 g/kg or 4 g/kg food) on high-fat diet-induced obesity and fatty liver formation in C57Bl/6J mice, and we examined their effects on calorimetry, metabolic performance, and the expressions of inflammatory genes and SIRT proteins. We found that resveratrol with 4 g/kg dose partially prevented hepatic steatosis and hepatocyte ballooning and induced skeletal muscle SIRT1 and SIRT4 expression while other examined parameter were unaffected by resveratrol. In contrast, CR provided superior protection against diet-induced obesity and fatty liver formation as compared to resveratrol, and the effects were associated with increased physical activity and ameliorated adipose tissue inflammation. CR increased expressions of SIRT3 in metabolically important tissues, suggesting that the beneficial effects of CR are mediated, at least in part, via SIRT3-dependent pathways.

Drugs influencing neurotransmission (alpha 1,2, beta 1,2 agonists, antagonists and diazepam) were given intraperitoneally to test their effects on the mortality of intravenous meglumine diatrizoate. Pretreatment with diazepam in two doses (0.14 and 0.56 mg/kg), alpha 1,2 agonist, beta 1 agonist and beta 1 antagonist affected LD50 so that 5 to 9 per cent more contrast medium could be administered to cause the same mortality as the contrast medium alone. Still higher (19-24%) doses could be used after an alpha 1,2 or alpha 1 antagonist. The beta 1,2 antagonist employed in the test decreased the contrast dosage by 13 per cent, causing the LD50.