A stable and catalytically active cathode consisting of homogeneously dispersed nano-socketed Fe–Ni particles has been elegantly fabricated in single-step treatment for solid oxide steam electrolysis cells via the in situ reduction of the Sr2Fe1.3Ni0.2Mo0.5O6 (SFMNi) material in a humidified H2 (3 vol% H2O) atmosphere at 800 °C.

No abstract

Here, we report the structure evolution, magnetic and ferroelectric properties in Co-doped 4- and 3-layered intergrowth Aurivillius compounds Bi4NdTi3Fe1-xCoxO15-Bi3NdTi2Fe1-xCoxO12-δ. The compounds suffer a structure evolution from the parent 4-layered phase (Bi4NdTi3FeO15) to 3-layered phase (Bi3NdTi2CoO12-δ) with increasing cobalt doping level from 0 to 1. Meanwhile the remanent magnetization and polarization show opposite variation tendencies against the doping level, and the sample with x = 0.3 has the largest remanent magnetization and the smallest polarization. It is believed that the Co concentration dependent magnetic properties are related to the population of the Fe3+ -O-Co3+ bonds, while the suppressed ferroelectric polarization is due to the enhanced leakage current caused by the increasing Co concentration. Furthermore, the samples (x = 0.1–0.7) with ferromagnetism show magnetoelectric coupling effects at room temperature. The results indicate that it is an effective method to create new multiferroic materials through modifying natural superlattices.

No abstract

No abstract

No abstract

Background:Previous studies[1] have shown that ATP acts on the sputum receptor P2X ligand-gated ion channel 7 (P2X7R) as a second signal to induce gouty arthritis.Objectives:In this study, the functional changes of three SNP genotypes, Ala348 to Thr, Glu 496 to Ala and Arg307 to Gln, in P2X7R were analyzed with high uric acid.Methods:After transfection of HEK-293T cells by lentivirus, observing the uptake ability of HEK-293T cells to ethidium bromide. The effect of three different mutants on the P2X7 receptor was thus observed on the P2X7 channel. In addition, THP-1 cells were also transfected, stable expression of a THP-1 cell line that has been transfected with a wild-type or different mutants and thus established. Then three types were set up separately, and each type was randomized into three groups: MSU(labeled M), MSU+ATP (labeled MA), and unstimulated control group (labeled C).Detection of IL-1β protein expression level in serum by ELISA and NLRP3, ASC and Caspase-1 mRNA levels in transfected THP-1 cells by qRT-PCR.Results:1.These three variants have different effects on the uptake function of ATP-induced ethidium+bromide in transfection of HEK-293T cells by lentivirus. Ala348to Thr increased P2X7-dependent ethidium+bromide uptake (145% of wild-type P2X7response, P<0.001). In contrast, Absent or very reduced P2X7function was found in Glu496to Ala and Arg307to Gln subjects, appeared to abolish P2X7-dependent dye uptake (38% and 32% of wild-type P2X7responses, P<0.001,), who were compared with wild-type.2.Compared the IL-1β levels of the three variants with the wide-type and empty virus in THP-1 cells, the Ala348to Thr mutation significantly up-regulated the serum levels of IL-1β compared with the wide-type and empty virus in group MA with high uric acid (P=0.0007;P=0.013, respectively). Moreover, similar results have also been shown in IL-1β mRNA expressions (P=0.0334; P=0.0307, respectively). The Glu496to Ala and Arg307to Gln mutations down-regulated the serum levels of IL-1β compared with the wide-type in group MA (P=0.0189;P=0.0164, respectively).3.NLRP3 mRNA was significantly increased in the Ala348to Thr mutation compared with the wide-type and empty virus in group MA (p =0.0003;P=0.0001, respectively). However, NLRP3 mRNA was significantly reduced in the Glu496to Ala and Arg307to Gln mutations compared with the wide-type in group MA (p =0.0294;P=0.0279, respectively).4.Wild-type was signifcantly higher than empty virus in the ASC gene expression in group MA(P=0.0022). Morever, the Ala348to Thr mutation was higher than empty virus while Arg307to Gln mutation was lower than that in group MA (P=0.0138;P=0.0283, respectively).5.Unlike NLRP3 gene expression, the data showed that the expression of caspase-1 mRNA in group C, M and MA all with no statistical significance, respectively (P>0.05).Conclusion:Our data revealed that Ala348to Thr up-regulate the functional status of P2X7R and Glu496to Ala and Gln460to Arg down-regulate the functional status of P2X7R, which resulted in a significant increase or decrease in IL-1β and NLRP3 expression levels with high uric acid.References:[1]Tao JH, Zhang Y, Li XP, et al. P2X7R: a potential key regulator of acute gouty arthritis[J].Semin Arthritis Rheum, 2013,43(3):376–380.Disclosure of Interests:None declared