women(190.60AE63.12 VS 205.40AE41.07), although the result was not statistically different. Besides, women with PCOS tended to have lower alpha and beta diversity in gut microbiota. Spearman correlation analysis showed that metabolic disorders were associated with changes in gut microbiota: 0h insulin, 2h insulin, and HOMA-IR values were negatively correlated with species richness (r¼-0.369,-.0425,-0.356, p<0.05). The content of genus Desulfovibrio in patients with PCOS was positively correlated with 0h glucose, 0h insulin, HOMA-IR, LDL (r¼0.485, 0.604, 0.640, 0.472, p<0.05). Genus Streptococcu was positively correlated with BMI, 1h blood glucose, 2h blood glucose, 2h insulin, HOMA-IR, cholesterol (r¼0.492, 0.485, 0.548, 0.477, 0.458, 0.516, p<0.05). CONCLUSIONS: Our study demonstrated that women with PCOS had lower biodiversity in gut microbiota, of which were closely associated with insulin resistance and abnormal lipid metabolism. Our findings suggest that changes in the gut microbiome may influence the development and pathology of PCOS and its metabolic disorders as well.