MECHANISM Against the background of blocking of sulfhydryl groups with cadmium sulfate, the contractile response of the isolated intestine and uterus of rats to neostigmine is inhibited. The response to acetylcholine and to barium chloride remains. Blocking of SH-groups of the intestine and uterus leads to the disappearance of the stimulant effect of the nicotine-like cholinomimetic DMPP. Blocking of nicotine-like cholinergic receptors with nikohexonium has a similar action on the effects of DMPP and neostigmine. The results obtained indicate the role of sulfhydryl groups of ganglionic nicotine-like cholinergic receptors in the mechanism of the stimulant effect of neostigmine on the intestine and uterus.Recognition of the leading role of the anticholinesterase effect in the mechanism of action of neostigmine and its analogs in no way rules out the possibility of their direct action on cholinergic receptors [3, 5, 8, 15, 27,29]. There is now weighty evidence to show that sulfhydryl groups are present in the structure of cholinergic receptors [6, 10, 11, 16, 23, 24], and also information concerning the role of these groups in the mechanism of action of cholinomimetics and cholinolytics with cholinergic receptors [4, 9,[12][13][14][19][20][21]The object of the present investigation was to study the role of the sulfhydryl groups of cholinergie receptors in the mechanism of action of anticholinesterase drugs.
EXPERIMENTALThe effect of neostigmine was studied in experiments on an isolated segment of ileum and on the isolated uterine cornu of albino rats.The animals were sacrificed immediately before the experiment. Contractions of the intestine and uterus were recorded on a kymograph.
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