Apicomplexan parasites are important pathogens of humans and domestic animals, including Plasmodium species (the agents of malaria) and Toxoplasma gondii, which is responsible for toxoplasmosis. They replicate within the cells of their animal hosts, to which they gain access using a unique parasite-driven invasion process. At the core of the invasion machine is a structure at the interface between the invading parasite and host cell called the moving junction (MJ) . The MJ serves as both a molecular doorway to the host cell and an anchor point enabling the parasite to engage its motility machinery to drive the penetration of the host cell , ultimately yielding a protective vacuole . The MJ is established through self-assembly of parasite proteins at the parasite-host interface . However, it is unknown whether host proteins are subverted for MJ formation. Here, we show that Toxoplasma parasite rhoptry neck proteins (RON2, RON4 and RON5) cooperate to actively recruit the host CIN85, CD2AP and the ESCRT-I components ALIX and TSG101 to the MJ during invasion. We map the interactions in detail and demonstrate that the parasite mimics and subverts conserved binding interfaces with remarkable specificity. Parasite mutants unable to recruit these host proteins show inefficient host cell invasion in culture and attenuated virulence in mice. This study reveals molecular mechanisms by which parasites subvert widely conserved host machinery to force highly efficient host cell access.

Objective: To determine whether earlier treatment of high-risk, prethreshold retinopathy of prematurity (ROP) improves retinal structural outcome at 2 years of age. Methods: Infants with bilateral high-risk prethreshold ROP had one eye randomly assigned to treatment with peripheral retinal ablation. The fellow eye was managed conventionally, and either treated at threshold ROP or observed if threshold was never reached. In patients with asymmetrical disease, the highrisk, prethreshold eye was randomised to earlier treatment or to conventional management. At 2 years of age, children were examined comprehensively by certified ophthalmologists to determine structural outcomes for their eyes. For the purposes of this study, an unfavourable structural outcome was defined as (1) a posterior retinal fold involving the macula, (2) a retinal detachment involving the macula or (3) retrolental tissue or ''mass'' obscuring the view of the posterior pole. Results of the 2-year examination were compared with those from the 9 months examination. Results: Data were available on 339 of 374 (90.6%) surviving children. Unfavourable structural outcomes were reduced from 15.4% in conventionally managed eyes to 9.1% in earlier-treated eyes (p = 0.002) at 2 years of age. Ophthalmic side effects (excluding retinal structure) from the ROP or its treatment were similar in the earlier-treated eyes and the conventionally managed eyes. Conclusion:The benefit of earlier treatment of high-risk prethreshold ROP on retinal structure endures to 2 years of age, and is not counterbalanced by any known side effect caused by earlier intervention. Earlier treatment improves the chance for long-term favourable retinal structural outcome in eyes with high-risk prethreshold ROP. Long-term follow-up is planned to determine structural and functional outcomes at 6 years of age.T he Early Treatment for Retinopathy Of Prematurity (ETROP) Study showed that retinal ablation for highrisk prethreshold retinopathy of prematurity (ROP) improved structural and functional outcomes, compared with conventional management, when infants were examined at 9 months' corrected age. 1 The study randomised infants who had both prethreshold disease and a risk for unfavourable structural outcome >15%. 2However, eyes of infants may change over time.3 Myopia, strabismus and late retinal detachments all increase in frequency in the months and years after successful treatment of ROP.3 4 In the Cryotherapy for Retinopathy of Prematurity (CRYO-ROP) Study, a wide distribution of optotype acuities developed after successful treatment, with 75% showing acuities worse than 20/40 when children reached 10 and 15 years of age.3 5 Time will tell whether the ETROP cohort will show the same distribution of functional outcomes as occurred in the CRYO-ROP Study.In the CRYO-ROP Study, only a few infants had threshold disease in zone I, 6 but in the ETROP Study, 40% of all randomised children had zone I disease. This group of children may be particularly vulnerable to complications of myopia, stra...

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The overall goal of this study was to examine the factor structure of the ''Experiences in Close Relationships'' instrument (Brennan et al. 1998; ECR) for assessing adult attachment among a clinical sample by conducting a series of exploratory factor analyses (EFA). While the measure demonstrated high reliability and strong validity among the original sample of college undergraduates (Brennan et al.), the factor structure had yet to be examined among participants involved in therapy. It is unclear if any results obtained using the ECR extrapolate to populations other than the original sample. Our findings support the factor structure of the ECR for assessment of attachment anxiety and avoidance among a more diverse, clinical population. Noteworthy differences between men and women also were found in the clinical sample. Finally, clinical implications for use with clients participating in therapy are discussed.

There is a well-established bidirectional, negative association between couple satisfaction and depressive symptoms. Yet, a family systems perspective emphasizes the role of the therapist in interrupting this recursive cycle between couple satisfaction and depressive symptoms. The current study utilized longitudinal data to explore the bidirectional associations between depressive symptoms and couple satisfaction, moderated by the therapeutic alliance over the course of therapy. The study included 108 couples participating in couple therapy at a university training clinic. Couples rated their depressive symptoms and couple satisfaction separately before the intake session and at the end of the fourth session, and they also reported their individual therapeutic alliance with the therapist at the end of the second and third sessions. Actor-partner interdependence moderation model analysis revealed several moderation effects. In general, with low therapeutic alliance, couples with higher initial symptoms (such as depressive symptoms and low couple satisfaction) reported more severe symptoms at the fourth session, compared to those who had fewer initial symptoms. The moderating effect of alliance on a couple's symptoms was found both among individuals, and between partners. Systemic clinical implications and suggestions for future research are discussed.

The present study examines adult attachment as a mediator, explaining the association between dyadic adjustment and depressive symptoms. Participants included 188 women and 35 men (N = 223) with a mean age of 28.9 years (SD = 9.95 years; range = 18–66 years). Results replicated previous research by demonstrating a significant association between poor relationship adjustment and depressive symptoms. However, the authors extended prior work identifying avoidance as a mediator of the relationship between each Revised Dyadic Adjustment Scale (RDAS) subscale (i.e., Satisfaction, Consensus, and Cohesion) and symptoms of depression. On the other hand, attachment anxiety mediated only the relationship between the cohesion subscale of the RDAS and depressive symptoms but was not influential in the association between satisfaction or consensus and symptoms of depression. Implications for the clinical treatment of depression among adults are discussed.

Interpersonal chemistry refers to a perceived instant connection that exists when meeting a person for the first time. In this study, we inductively explored the characteristics of friendship and romantic chemistry for men and women. A social constructionist lens guided our data collection and interpretation. The sample consisted of 362 ethnically diverse individuals (men = 162, women = 200) who completed an online survey. Using textual coding methods, we identified the core themes for interpersonal chemistry as: Reciprocal candor, mutual enjoyment, attraction, similarities, personableness, love, instant connection, and indescribable factors. The similarities theme was more characteristic of friendship than romantic chemistry and the attraction and love themes were more salient to romantic chemistry. We analyzed the data separately for men and women and found that women’s responses centered more on love as characteristic of friendship chemistry and similarities as a component of romantic chemistry.