The production of proteolytic enzymes by two strains of <i>Beauveria bassiana</i> 278, <i>B. bassiana</i> 446 and one strain of <i>Ascosphera apis</i> 496 was analysed. It was demonstrated that the strain of <i>B. bassiana</i> 278 proved to be the best producer of basic and acid proteases. The influence of different environmental factors such as nitrogen and carbon sources on the production of extracellular hydrolytic enzymes was assessed. In addition the acid protease from <i>B. bassiana</i> was partially characterized.
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