Microcystin toxin-producing cyanobacteria are known to have harmful effects on humans and animals. We have developed a loop-mediated isothermal amplification (LAMP)-based detection method by targeting the microcystin synthetase B gene (), the gene responsible for the production of microcystin. The sensitivity of the method was found to be 1 fg per reaction, and it was 1000-fold higher than the conventional PCR. The LAMP method was able to amplify the target gene with a minimum amount of dNTP (0.4 mM), which further reduces the cost of reaction. The improved LAMP assay could detect the presence of the toxin-producing cyanobacteria in water samples within 2 h of time, which demonstrates the rapidness of the method. Freshwater samples were screened using the developed LAMP, and seven water samples collected from lakes and a bird sanctuary tested positive for gene harboring cyanobacteria, and negative in all other drinking waters. Hence, the developed LAMP could be a possible alternative to the existing molecular methods for screening for microcystin in environmental samples with greater sensitivity.
The massive deployment of unmanned aerial vehicles (UAVs) is growing in various fields in wireless network of 5G and 6G. It supports huge deployment of UAVs that offer secure, reliable, and low-cost data communications. They are widely deployed in surveillance and military applications for collecting sensitive and critical data, transferring the collected data to the data center, procuring high-definition images of disaster areas, surveying natural disaster zones, and other critical missions. Although, the UAV applications are growing, the risk and security factors associated with it are increasing parallelly. So, a need of secure and reliable network communication system for UAV is mandatory. In this chapter, the performance of UAV network system in 6G environment and the technological challenges faced with respect to security and privacy are discussed. Then a possible solution using blockchain and security policies that can be provided for a secure communication in a 6G network is presented.
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