SynopsisA radioimmunoassay (RIA) method for luteinizing hormone (LH) releasing hormone (RH) utilizing rabbit antiserum against synthetic (Glu1)-LH-RH coupled with human serum albumin at the N-terminus, is described. This assay system for LH-RH also cross-reacted with several LH-RH analogues or fragments, but not with pituitary trophic hormones.The assay was performed on the hypothalamic extracts of adult ovariectomized rats and female immature rats which had been treated with estradiol.The FSH and LH levels in the pituitary gland and serum of the same animals were determined by RIA. The radioimmunoreactive LH-RH content of the stalk median eminence markedly increased seven days after ovariectomy.The serum levels and the pituitary contents of FSH and LH of the same rats were also significantly augmented.In immature rats, the hypothalamic content of LH-RH, as measured by RIA, was significantly increased one hour after the injection of estradiol. The FSH and LH levels in the pituitary showed a significant rise after 7 hours.
Serum levels of testosterone (T), dihydrotestosterone (DHT), androsterone (A), 5α‐androstane‐3α, 17β‐diol (5α‐diol) and estradiol‐17β (E2) were measured by radioimmunoassay in the sera of 9 patients with untreated prostatic cancer and in 11 with benign prostatic hypertrophy (BPH). Basal levels and responses to hCG stimulation were investigated. Although no specific changes in steroid hormone levels in either disease group were found, response patterns of serum T, DHT, and E2 were shown to be those characteristic of male senescence, suggesting a relative predominance of estrogens over androgens. Neither 5α‐diol nor A exhibited appreciable responses to hCG stimulation in our study.
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