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Purpose To evaluate the measurements reproducibility of the macular area and of the retinal nerve fiber layer (RNFL) taken with different protocols with swept source optical coherence toography (SS‐OCT) Triton. Methods 108 eyes from 108 healthy subjects were included whose macular thickness and RFNL thickness were analyzed by SS‐OCT Triton (Topcon, Japan). To study the reproducibility of the device, three measurements were taken followed by different protocols: 3D (H) + 5LineCross and 3D Macular (H) protocols (macular thickness) and 3D Disc protocol (RNFL and optic nerve morphometric values). The coefficients of variation (COV) and intraclass coefficients (ICC) were calculated. Results A high reproducibility was obtained with mean COV by type of protocol used of 8.31 ± 13.54% to 1 ± 1, 6% being the less reproducible protocol for the measurement of RNFL 8.31 ± 13.54%. The macular area data in all ETDRS quadrants (11.6% to 37%) were more accurate than those of RNFL (2% macular vs 8% RNFL). The ICC of the optic nerve morphometric values presented a range of 0.91 to 0.53, being the vertical diameter disk value the less accurate. The ICC values of the total macular thickness were the most accurate (0.963 to 0.839). Little variability was observed in the RNFL thickness (ICC 0.946 to 0.750). Conclusions There is a high reproducibility of the protocols performed with Triton OCT. The total value of the RNFL protocol is much more variable than the macular protocols, being the temporal quadrants less reproducible than the nasal ones. These results could influence negatively in the evaluation of patients with neurodenegerative diseases where the most affected area is the temporal one.