To facilitate eventual genetic vaccination of mucosal tissues, a receptor-mediated gene transfer system was devised using the reovirus adhesin, protein 1. Highly efficient uptake and internalization of protein 1 polylysine (PL) DNA complexes could be demonstrated by fluorescent microscopy. Successful cellular transfection of rodent and human cell lines was obtained with the recombinant protein 1 as a PL-DNA complex, and could be shown to be receptor-specific. Transfection efficiency was dependent upon the
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