A simple, accurate, and precise method for estimating Aspirin, Atorvastatin, and Ramipril in Tablet dosage form was developed. Symmetry C18 (150 x 4.6 mm, 3.5) was used to run the chromatogram. At a flow rate of 1.0 ml/min, a mobile phase containing potassium dihydrogen ortho phosphate (pH adjusted to 3) and Methanol in the ratio 35:65 was pumped through the column. The temperature was kept at 30°C. The optimised wavelength chosen was 240.0 nm. Ramipril, Atorvastatin, and Aspirin had retention times of 1.803 minutes, 2.506 minutes, and 3.875 minutes, respectively. The percent RSD of Ramipril, Atorvastatin, and Aspirin were determined to be 0.99, 0.42, and 0.42, respectively. The method is linear for Ramipril concentrations ranging from 12.5 to 62.5 g/ml, Atorvastatin concentrations ranging from 160 to 800 g/ml, and Aspirin concentrations ranging from 5 to 25 g/ml. The method was accurate, with a percent recovery of 99.13 to 100 percent for Ramipril, 99.3 to 100.2 percent for Atorvastatin, and 99.3 to 100 percent for Ramipril, respectively. The developed method is robust after deliberate changes in flowrate and mobile phase composition with a percent RSD less than 2.
A specific, linear and accurate LC-MS/MS method was developed for the determination of febuxostat
in human K2 EDTA plasma and it was successfully applied for the pharmacokinetic study in wister
rats. Chromatographic isolation of febuxostat and febuxostat D9 were attained on Purosphur C18, 100
× 4.6 mm, 5 μ column with 1.0 mL/min flowing rate. The technique was linear over the standard
concentrations ranging from 24.995-7001.401 ng/mL and the regression coefficient was perceived to
be ≥ 0.9997. All LLOQ samples % RSD of back computed concentrations varied from 1.00 to 4.01.
The analysis of plasma from healthy rats was successfully conducted using the validated LC-MS/MS
procedure to quantify the presence of febuxostat. From the pharmacokinetic studies, Tmax, Cmax, T1/2
and AUC0-∞ of the febuxostat tablets were 2.0 ± 0.03 h, 8.85 ± 1.87 ng/mL, 6.34 ± 0.53 h, 95.58 ± 6.37
ng h/mL, respectively.
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