Porcine and ovine follicular tissues were used to investigate, in vitro, the effect of charcoal-treated aqueous extract from ovine corpora lutea of pregnancy on aromatase activity as determined by the conversion of [3H]testosterone to oestradiol by follicular walls and measurement of 3H2O release. Extract (500 micrograms protein) prepared from corpora lutea of day 112 of pregnancy but not extract (500 micrograms) prepared from ovine fetal cotyledonary tissue obtained at a similar time significantly decreased (P < 0.02) aromatase activity of pig follicles in the absence of FSH. These results demonstrate that a non-steroidal factor in the corpora lutea of late pregnancy directly inhibits aromatase activity. When the effects of different doses (300, 600 or 1200 micrograms) of luteal extract from corpora lutea of day 100 of pregnancy on aromatase activity of pig follicles were studied, the dose by treatment (presence or absence of FSH) interaction was not significant. Luteal extract dose at 300 micrograms did not affect aromatase activity but a significant decrease in activity occurred at 600 micrograms of luteal extract (600 versus 300 micrograms, P < 0.02). There was no further significant increase in the inhibitory effect with 1200 micrograms luteal extract. When the effects of 600 micrograms luteal extract from corpora lutea of days 15, 75 or 100 of pregnancy on aromatase activity of pig follicles were studied, a significant (P < 0.05) stage of pregnancy effect was detected, but the stage of pregnancy by treatment (presence or absence of FSH) interaction was not significant. No effect was noted with day 15 or day 75 luteal extract.(ABSTRACT TRUNCATED AT 250 WORDS)
Abstract1 Das Kondensationsprodukt (I) von 6‐Amino‐1,3‐benzodioxan mit Äthoxymethylenmalonsäurediäthylester (ÄMD) gibt nach Cyclisierung mit POCl3 als Hauptprodukt das Chinolin (II).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.