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Antialia liadin antibodies(AGA)are a usefil tool in screenin Coeliac fiisease (CD).Anyway this test requires a rather specfa:?: zed 1aboratory.a venous bloAq sam le and about one week in order to obtain the result for the' famiyy" paediatrician.We developed and evaluated a dot immunobinding assay to detect alfagliadin IgG and IgA antibodies in a single dro of whole blood The method 1s based on the absorption of alfa-geiadin as a spot'on to nitrocellulose sheets yho are immobilized on plastic strips.The strips were incubated 3b with atient szmple,and 30' with alkaline fk;:gm:a;;(i;:jufate ant3human (IgA and 1gC)antibodies.After short w th the substrate solution,the strip was dried and finally examined for the rasults,which are ex lessed by a,colorimetric reaction Twent patients wlth CD(10 w?;h active dlsease, 10 during gluten kree d%et(~F~)), 11 children with other gastro

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