The aim of this study was to demonstrate the antioxidant effect of selenium in its ordinary and Nano form on oxidative stress induced by iron overload in male rat. This study was carried out on 90 rat, weighted 180-200 gm. Rats were classified into six groups Group 1: control group. Group 2: Nano selenium-treated group. Group 3: selenium treated group. Group 4: iron overload treated group. Group 5: iron overload + Nano selenium-treatment. Group 6: iron overload + selenium-treatment. Blood and liver tissue samples were collected from all groups after 1, 3 and 6 weeks from the onset of the experiment. Serum used for measurement of total iron. Liver tissue was processed for measurement of molonadialdhyde (MDA), total antioxidant capacity (TAC), glutathione peroxidase (GSH-PX), super oxide dismutase (SOD), glutathione reduced (GSH) and Catalase. The obtained results revealed that, Nano-selenium + iron and selenium + iron-treated groups lead to decrease the concentration of MDA, total iron and significant decrease in GSH-PX-1 mRNA expression and increase the concentration of TAC, catalase, GSH and SOD when compared with iron overload treated group. From the result, it can be concluded that selenium in its ordinary and Nano forms have antioxidant and hepatoprotective effect in experimentally-induced iron overload in male Wister rats. Nano form has superior effects than ordinary form of selenium.

This study was performed on fifteen newly born calves, 3-5-month-old with clinical signs of bronchopneumonia in addition to five clinically healthy calves which considered as control group. After bacterial isolation and identification of the causative microorganisms, fifteen calves from which was found infected with Pasteurella spp. and Haemophilus spp. The diseased calves were divided randomly into three equal groups which respectively, treated with: Gp.1: were treated dose of draxxin (2.5 mg/kg body weight) by s/c injection in the neck; Gp.2: were received Draxxin and Meloxicam (single I/M injection of 0.5 mg/kg b.wt.), while Gp.3: were received draxxin (single s/c injection of 20 mg/kg b.wt.) +Symopro on drinking water (150-300 ml/1000 L for 5 days). Blood and serum samples were collected from all calves just before treatment, 3 and 10 days post treatment. pH, PO2 and SO2 results recorded significant increase in all treated groups. Meanwhile, PCO2, tCO2 and HCO3 results showed significant decrease in all treated groups compared with diseased group before treatment. Haptoglobin and fibrinogen results showed significant decrease in all treated groups compared with diseased group before treatment. GSH-PX results revealed significant increase. Meanwhile, MDA results showed significant decrease in all treated groups which comparatively with those before treatment. There was significant increase in cellular immunity (phagocytic index and phagocytic%) in treated group with draxxin plus sympro when compared with diseased group before treatment.

This study was performed on fifteen newly born calves, 3-5 month old with clinical signs of bovine respiratory disease (BRD) in addition to five clinically healthy which considered as control group. After bacterial isolation and identification of the causative microorganisms, fifteen calves from which were found infected with E.coli, P. multocida, Klebsiella and S. aureus. The diseased calves were divided randomly into three equal groups: Gp.1: were treated dose of draxxin (2.5 mg/kg body weight) by s/c injection in the neck; Gp.2: were received Draxxin and Meloxicam (single I/M injection of 0.5 mg/kgb.wt.), while Gp.3: were received draxxin (single s/c injection of 20 mg/kg bwt.) +Levamisole (single I/M injection at a dose of 1mg/50kg bwt). Blood and serum samples were collected from all calves just before treatment, 3 and 10 days post treatment. TLC, neutrophils and monocyte count revealed significant decrease in all treated groups in comparison with diseased group before treatment. While, lymphocyte count recorded significant increase in all treated groups compared with diseased group and lastly, esinophil recorded significant decrease in treated groups with draxxin plus meloxicam and draxxin plus levamisole in comparison with diseased group. Total protein demonstrated significant increase in all treated in comparison with diseased group. Serum albumin, total globulin, α-globulin, β-globulin and γ-globulin showed significant decrease when compared with diseased calves before treatment. Haptoglobin and fibrinogen showed significant decrease in all treated groups in comparison with diseased group. There were significant increase in immunoglobulins and cellular immunity (phagocytic index and phagocytic percentage) in all treated groups especially in group treated with draxxin plus levamisole when compared with diseased group before treatment.

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