Eksplorasi imbuhan pakan organik untuk ternak saat ini terus dilakukan untuk menggantikan antibiotik sebagai pemacu pertumbuhan. Probiotik dari bakteri asam laktat telah digunakan secara luas untuk mendukung keseimbangan mikroba saluran pencernaan. Selain itu, mineral organik ditambahkan pada pakan untuk meningkatkan ketersediaan dalam mencegah kelainan metabolisme akibat defisiensi mineral. Penelitian ini bertujuan untuk mengevaluasi penambahan probiotik (Pediococcus acidilactici RS2) dan mikromineral terkorporasi khamir/micromineral enriched yeast (MEY) yang dikombinasikan dengan tanin dari daun mimba (Azadirachta indica) terhadap fermentabilitas rumput Raja (P. hybrid) dengan menggunakan teknik produksi gas in vitro. Perlakuan terdiri dari P0 (kontrol/hijauan tanpa imbuhan), P1 (P0+MEY); P2 (P0+MEY+tanin); P3 (P0+Probiotik); P4 (P0+Probiotik+MEY), dan P5 (P0+Probiotik+MEY+tanin) yang disusun dalam rancangan acak lengkap dengan 3 ulangan setiap perlakuan. Suplementasi MEY (P1), probiotik+MEY (P4) dan probiotik+MEY+tanin (P5) nyata (P<0,05) meningkatkan produksi gas hijauan tanpa berpengaruh pada asam lemak volatil (VFA), jumlah protozoa, produksi metana dan kecernaan in vitro. Produksi gas tertinggi tercatat pada hijauan yang diberi perlakuan P4 diikuti P5, P1, P2, P3 dan kontrol. Pengaruh perlakuan terhadap kinetika produksi gas menunjukkan perbedaan yang nyata pada inkubasi setelah 8 jam. Walaupun perlakuan hanya berpengaruh terhadap kinetika produksi gas (b, c dan total gas), berdasarkan analisis kluster-hirarki menunjukkan beberapa parameter yang terdiri dari asetat, propionat, kecernaan in vitro, jumlah protozoa dan produksi gas metana sangat berkorelasi dengan parameter kinetika produksi gas. Dapat disimpulkan bahwa fermentabilitas hijauan dapat ditingkatkan dengan suplementasi mineral organik maupun kombinasinya dengan probiotik maupun probiotik+tanin tanpa berpengaruh negatif terhadap kecernaan in vitro.
A simple method for the preparation of a specimen for observing Saccharomyces cerevisiae B-18 by using a high vacuum mode setting of scanning electron microscopy (SEM). The method without chemical fixatives (F0) was very simple, the culture was directly taken from Chloramphenicol Yeast Glucose Agar (CYGA) by using a loop needle and coated with a film of gold (Au) without using any chemical fixative and only air dehydrating procedures. The method with chemical fixatives (F1) like osmium tetroxide (OsO4), is highly toxic and corrosive. The methods also take much time for the preparation of samples before observation. The morphology of S. cerevisiae B-18 was observed of two magnifications, 5K and 10 K and over a range of time setting of ion sputter and one current that is ten mA. The treatments of ion sputter current exposure in this study were ten mA for 10 (T10), 20 (T20), 30 (T30), 40 (T40), 50 (T50), and 60 (T60) seconds. This preparation procedure could be most useful for S. cerevisiae morphological screening of rapidly and safely than using chemical fixatives before using adequate preparation procedure with chemical fixatives and could substitute light microscopy method with better magnification. The best result is the ion sputter setting ten mA for 60 seconds (T60) without any charging phenomenon disturbances. We have suggestions for F0; the first is the maximum duration of the sample that had air-dried is about three hours because more than its period, the resolution would be not proper. The optimum period for observation in the chamber in the high vacuum condition is about two hours and the second to developing methods so that representation for cell diameter measurements can be even better than this study.
Probiotic and prebiotic or their combinations can potentially function as an alternative to antibiotics growth promoters (AGPs) for broiler. This study was designed to investigate the growth performance, intestinal microstructure, and nutrients digestibility of broilers administered with probiotics of Lactobacillus plantarum AKK30 and Saccharomyces cerevisiae B18 in combination with inulin. A total of 275 male chickens (initial bodyweight of 47±0.05 g) were reared for growth performance evaluation. At the 32-d-old, 25 male chickens were necropsied for intestinal microstructural analysis, while the other 25 male chickens were selected for evaluation of digestibility (body weight= 1525±0.08 g). Treatments of probiotics in combination with different levels of inulin consisted of control with probiotics without inulin (S0), probiotics with 0.5% of inulin (S1), probiotics with 1.0% of inulin (S2), probiotics with 1.5% of inulin (S3), and commercial probiotics without inulin (Sc), which were arranged in a completely randomized design with five replications. Results showed that body weight gain and performance index in broilers treated prebiotics in combination with 0.5% inulin (S1), 1% inulin (S2), and commercial probiotic without inulin (Sc) were significantly higher (p<0.05) than those in control broiler chickens that were treated with probiotics without inulin. Feed intake showed no differences among treatments, whereas feed conversion ratios in broiler chickens treated with the commercial probiotics (Sc) or probiotics in combination with inulin at 0.5% (S1) and 1.0% (S2) were lower than control chickens. Metabolizable energy, nitrogen retention, and villi height in chickens treated with probiotics in combination with 0.5% inulin (S1) and 1.0% inulin (S2) were higher than those in the control group. In conclusion, the administration of probiotic combined with inulin at the level of either 0.5 or 1.0% improves broiler performance, intestinal microstructure, and nutrients digestibility.
Lactobacillus plantarum AKK30 inoculum in de Maan Rogosa Sharpe Broth (MRSB) grown on media containing oligosaccharides (inulin or mannan oligosaccharides/MOS) was studied in vitro and in vivo. In vitro assay was conducted to characterize metabolite profiles and its effect on pathogenic bacteria, while in vivo assay was conducted to study the metabolic energy, nitrogen retention, and intestinal morphology of broilers. A total of 24 male broilers-40-day-old (average body weight= 1.725±0.05 kg) strains Cobb-500 were used, where 20 birds were added 1% inoculum L plantarum AKK30 of daily intake and randomly distributed in five treatments and four replications consisted of: A) MRSB + L. plantarum AKK-30 (10⁷ cfu g -1 ); B) MRSB + inulin 0.5% (w v -1 ); C) MRSB + MOS 0.5%(w v -1 ); D) L. plantarum AKK-30 (10⁷ cfu g -1 ) + MRSB + inulin 0.5% (w v -1 ).; and E) L. plantarum AKK-30 (10⁷ cfu g -1 ) + MRSB + MOS 0.5% (w v -1 ), while 4 birds were fasted as endogenous chicken. The results showed that the highest isoleucine and threonine were found in group E. The highest oleic acid and the lowest conjugated linoleic acid (CLA) were observed in all treatments and inoculum did not affect antibacterial activity. The metabolizable energy values and nitrogen retention in group D and E were higher than other treatments (p<0.05). The lowest height of villi was observed in group A (p<0.05). In conclusion, the addition of oligosaccharides inulin or mannan oligosaccharide (MOS) affects the metabolite profiles of Lactobacillus plantarum AKK30 and their synbiotic effects improve intestinal morphology, metabolizable energy, and nitrogen retention in broilers.
Phytic acid is an anti-nutrition substance due to its ability to bind minerals such as Mg, Fe, Zn, Mn, Ca, and enzyme proteins resulted in decrease in mineral solubility. Phytic acid levels can be reduced by phytase [myo-inositol hexacryphosphate phosphohidrolase]. Microbial phytase can come from fungi, bacteria, and yeast sources. This research was conducted with the aims to evaluate the effect of probiotics phytase-producing supplementation as feed additive on nutrient digestibility of broiler. This study was performed in a completely randomized design for the environmental design, which consist of five treatments. Negative control (without probiotics phytase-producing), LAB (Lactobacillus plantarum A1-E) phytase-producing, yeast (Candida tropicalis TKD-3) phytase-producing, probiotic consortium (L. plantarum A1-E and C. tropicalis TKD-3) and positive control (commercial probiotic). The observed variables are feed intake, energy intake, excreta weight, energy excretion, nitrogen intake, nitrogen excretion, nitrogen retention, apparent metabolizable energy (AME), true metabolizable energy (TME), apparent metabolizable energy corrected for nitrogen (AMEn) and true metabolizable energy corrected for nitrogen (TMEn). Data were analyzed by using analysis of variance (ANOVA) and followed by Duncan’s multiple range test to distinguish the effect of different treatment mean. The results of variation analysis on AMEn and TMEn showed significant different while another parameter showed no significant difference between treatments. The use of C. tropicalis TKD-3 as probiotics phytase-producing on broiler chicken diet increase nutrient digestibility, especially apparent metabolic energy corrected nitrogen, and true metabolic energy corrected nitrogen.
Abstract. Suryani AE, Anggraeni AS, Istiqomah L, Damayanti E, Karimy MF. 2021. Isolation and identification of phytate-degrading yeast from traditional fermented food. Biodiversitas 22: 866-873. Application of phytase (myo-inositol hexakisphosphate phosphohydrolase) to catalyze the release of phosphate from phytates contained on grain-based feed has been used widely in poultry feed industry. In this study, yeast as phytase producer from traditional fermented food was isolated, screened and identified their morphological, biochemical, and molecular characteristics. Production of extracellular phytase from yeast was quantified using spectrophotometer. The results showed that among 8 yeast isolates that had phytase activity, there were two isolates with the highest phytase activity and specific activity which were TKd3 isolate (6.57 U/mL and 54.230 U/mg) and GF1 (6.07 U/mL and 53.68 U/mg). Morphological identification using Scanning Electron Microscope revealed that TKd3 cells isolated from soybean tempeh had an elongated oval cell structure, whereas the GF1 isolated from fresh gatot had a rounder cell structure. TKd3 isolate with accession number MW131530 had homology with Candida tropicalis ATCC 750 28S rRNA with 99.83% similarity and GF1 isolate with accession number MW131531 had homology with Candida tropicalis ATCC 750 28S rRNA with 100% similarity. It could be concluded that C. tropicalis yeast from traditional fermented food produced the extracellular phytase for further use of phytase in poultry feed additive.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.