This study was conducted to compare the effects of different types and levels of organic acids being Formic (0.25, 0.5 and 1.0% FA), Fumaric (0.5, 1.0 and 1.5% FUA), Acetic (0.25, 0.50 and 0.75% AC) and Citric Acids (1, 2 and 3% CA). A total number of 351 one d-old Arbor-Acres broiler chicks were randomly divided into 13 treatment groups, each in three replicates of 9 chicks per replicate. The first group was fed the basal diet without supplementation and served as control. While, the other groups were received the basal diet supplemented with the tested organic acids. The experiment was lasted when chicks were 42 d old. Performance, pH of some gastrointestinal tract (GI-tract) segments, organ morphology, cecal bacteria, blood metabolites and nutrients digestibility were measured. Results obtained could be summarized as follow: 1-Any of the tested organic acids increased significantly (p<0.01) body weight gain and European Production Efficiency Index compared with the control group. However, feed conversion ratio improved, but not significantly in case of formic and fumaric acids, while significantly improved with acetic and citric acids supplementation, compared to the control. No great differences in mortality rate were observed among all treatments as from 1 to 2 birds were died all over the experimental period due to natural causes and not due to nutritional treatments. 2-Dietary organic acids improved both ME and nutrients digestibility of the experimental diets compared to the control. 3-Dietary organic acids significantly reduced (p<0.01) the pH values in the different GI-tract segments of the chicks compared to chicks fed the control. 4-Feeding dietary FA and FUA led to sharp reduction in caeca content of lactobacilli bacteria, but increased number of coliforms bacteria and anaerobes was slightly increased. Feeding 0.75% AC and 2%CA increased Lactobacillus count and coliforms. Villus height was significantly higher (p<0.01) of all organic acid supplementation fed groups. 5-Blood serum content of calcium, phosphorus, total protein and globulin were increased significantly (p<0.01) by 0.5% FA, 0.5% FUA, 0.75% AC and 2%CA in the diet compared to the control. 6-Relative weights of lymphoid organs (spleen, bursa of fabrics and thymus gland) were significantly higher (p<0.01) by supplementing the tested organic acids compared to the control group. In conclusion, dietary 0.5% either of formic or fumaric as well as 0.75% acetic or 2% citric acids could be used safely to improve performance and health of broiler chickens.
he objective of this study was to investigate the effect of dietary sumac seed powder (SSP) as an antioxidant and growth promoter on growth performance, carcass characteristics and blood constituents. A total number of 240 Japanese quail birds 7 days old were distributed into 4 groups (n=60) with 3 replicates (20 in each). The first group was fed the control (0.0% SSP), while, the other groups fed 1.5, 2.0 and 2.5% SSP. Body weight and feed consumption were recorded biweekly, while, mortality rate was recorded daily. Average body weight gains, feed conversion ratio, and European Production Efficiency Index (EPEI) were calculated while; bacterial count of intestinal contents was estimated. At the end of the experiment, carcass characteristics were measured, moreover, blood samples were taken to determine some blood constituents and organoleptic evaluation of cooked chicken meat. Results indicated that quail chicks fed different levels of SSP had the heaviest (p<0.05) body weight and body weight gain and better FCR and EPEI as compared to the control group. Sumac Seed Powder with different levels significantly (p<0.05) improved dressing, breast, total edible parts and the values of the meat quality including taste, aroma, texture, color and overall acceptability compared to the control group. Abdominal fat percentage was absent with different levels of sumac compared to control group. Chicks fed 2 and 2.5%SSP showed significantly (p<0.05) higher spleen, bursa and thymus. The ALT, AST, cholesterol, LDL, glucose, A/G and the population of E.coli of the control were higher than the birds fed SSP. Total antioxidants capacity, total lipids and globulin of the sumac fed groups were higher than the birds in control. Total plasma protein was significantly higher with 2%SSP compared to other treatments. Generally, diets supplemented with sumac seed powder (1.5, 2 and 2.5%) significantly improved growth performance, carcass, meat quality and plasma blood constituents in Japanese quail. The best supplemented level of SSP was 2%.
Infectious Bronchitis (IB) is currently one of the most important diseases in poultry flocks all over the world causes huge economic losses in poultry industry. Many IBV outbreaks associated with respiratory distress, nephropathy, and high mortalities were attributed to the circulation of new nephropathogenic IBV variant 2 strains. This study was conducted to development of bivalent inactivated IBV vaccine by using the local classical and variant isolates (KP279995/2014 and KP279998/2014 respectively) which isolated from poultry farms in Egypt as previous surveillance study during IBV outbreak from Al-Sharkia and Al-Qalubia governorates. The locally prepared vaccine formulated by using formalin for inactivation and Montanide TM ISA71 RVG as oil adjuvant. The prepared bivalent inactivated (IBV) vaccine was tested for sterility, safety and potency, the efficacy of the prepared vaccine was applied in specific pathogen free chicks (SPF) for monitoring the antibody titers by using of Enzyme Linked Immuno Sorbent Assay (ELISA) post vaccination with estimation of shedding parameters by Real-Time RT-PCR in challenging birds with 10 4.5 EID 50 /dose challenge IBV strains (classical and/or variant). The results revealed that the prepared vaccine free from any bacterial or mycotic contamination also safe after double dose inoculation in SPF chicks. The efficacy of inactivated vaccine showed from 92% to 96% protection against homologous challenge based on assessment parameters. This confirms that poultry industry can be protected from IB disease if using locally isolates in preparing of inactivated vaccine which reduce the economic losses caused by IB infection viruses in Egypt. ـــــــــــــــــــــــــــــــــــــــــ
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