This work was carried out in collaboration between the authors. Author KTM carried out the sample collection, designed the study template, managed the literature searches and prepared the manuscripts for publication. Author MEA carried out the biochemical analysis, statistical analysis, and discussion of results. The authors read and approved the final manuscript.
Objective: To evaluate microbial quality assessment of street vended soyabean cheese products (Tofu) sold in Minna metropolis
Method: Standard microbiological pour plate technique was used to examine the microbial content of ready to eat soybean cheese and also to isolate, characterize and identify the microorganisms.
Result: The results revealed the viable bacterial counts ranged from 1.40 x 105 cfu/ml - 8.40 x 105 cfu/ml, enteric bacterial counts ranged from 1.10 x 105 cfu/ml – 7.60 x 105 cfu/ml and fungal counts ranged from 3.0 103 cfu/ml – 36.0 x 103 cfu/ml. The bacteria isolated from the samples were Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa and species of Klebsiella and Bacillus while fungi isolated include Microsporum canis, Trichophyton rubrum, Aspergillus niger and species of Candida and Mucor. The most frequently occurring bacteria was S. aureus while the most frequently occurring fungal was species of Mucor.
Conclusion: The results suggested that the soybean cheese samples were contaminated with pathogenic microorganisms and could cause health hazard to the consumers. There is need to improve personal hygiene and environmental sanitation as a good hygienic practice in the production and preparation of the soybean cheese since it serves as a good source of protein, carbohydrate and fat being of great nutritional value to the consumers.
The antibacterial effect of Azadirachta indica against Pseudomonas aeruginosa, Klebsiella ozanae, Staphylococcus aureus and Escherichia coli was determined using the agar cup plate technique. The phytochemical components of A. indica showed the presence of saponin and phlobatanin and the absence of alkaloids, tannins, phenolics, glycosides, flavonoids and triterpenes. The result showed that the test organisms were susceptible to 500mg/ml, 50mg/ml and 5mg/ml of the plant extract. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The result showed that the MIC and MBC were 5mg and 50mg respectively for Pseudomonas aeruginosa, Kl. ozanae, Staphylococcus aureus and Escherichia coli. The result of the study suggests that extracts of A. indica could be suitable for the treatment of various infections caused by P. aeruginosa, K. ozanae, S. aureus and E. coli.
The antibacterial assay of the leaf of Telfairia occidentalis (Fluted pumpkins) on Salmonella typhi, Escherichia coli and Streptococcus faecalis was determined using the agar diffusion technique to investigate its potential use as antibacterial agent. The Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and phytochemical components of the leaf were studied. The phytochemical screening of the extract indicated the presence of glycosides, saponins, flavonoids, phenolics and steroids. The extract showed a higher antibacterial activity against E. coli (20 ±0.58mm at 500mgmg.ml), S. faecalis (6 ± 1.10mm at 5.0mg) and S. typhi (11±0.70mm at 50mg/ml). The MIC were 0,5mg/ml, 5.0mg/ml and 500mg/ml for E. coli, S. typhi and S. faecalis respectively. The MBC for E. coli and S. typhi were 0.5mg/ml and 5.0mg/ml respectively while S. faecalis was resistant to the extract. The result of the study suggests that leaf extract of T. occidentalis can be used for the treatment of infections by the test organisms.
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