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In order to dissect the complex genetic system that controls pollen development, we have undertaken a program of transposon insertion mutagenesis, with the purpose of producing mutations in gametophytically acting genes that are important for this process. The present work reports the developmental cytology of one of the mutants isolated, gaMS-2 (gametophytic male sterile-2). A peculiar feature of the mutant grains was lack of differentiation between the vegetative and the generative nuclei, leading to alteration in number, conformation and placement of nuclei. At anthesis, the grains carrying the mutant allele are about 40% of the normal grain size, contain a very reduced amount of starch and exhibit various nuclear abnormalities.& k w d : Key words Maize · Pollen · Male sterility · Gametophytic mutants& b d y :

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