gene expression were performed. RNA was extracted from treated and untreated cowpea embryos and seedlings at different time points after imbibition and cDNA was synthesised. The ceramide synthase gene was amplified using primers designed to conserved gene regions of legume plants. Sequenced analysis of the cowpea ceramide synthase gene fragment confirmed the primers amplified the correct gene. Future work will examine the expression of the ceramide synthase in FB 1 treated cowpea seeds.
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