Horizontal starch gel electrophoresis was applied to the study of almond identification [Prunus amygdalus Batsch, syn. P. dulcis (Mill.) D.A. Webb] using pollen from 44 cultivars of different origin. Variability was found in all of the nine enzymatic systems analyzed (PGM, AAT, GPI, LAP, 6PGD, AcP, CAT, IDH, and ADH). The enzymatic systems with the highest discriminating possibilities were PGM, CAT, AcP, and LAP. This variability enabled the 44 cultivars to be distinguished with the exception of two pairs.