Albumen from hen eggs was diluted 3-fold with 0.05 M NaCl solution at pH 4.0 and was further treated with 30% ethanol for 8 h. The supernatant (77900 U/mg protein) thus obtained was further diluted (2.5-fold) with distilled water and its pH value was adjusted to 8.0 before being subjected to alcohol-insoluble cross-linked pea pod solid (AI-CLPPS) ion-exchange chromatography for lysozyme isolation. Results showed that AI-CLPPS ion-exchange chromatography increased the purification to 68-fold with a 72% lysozyme recovery from the starting albumen.Key Words: hen egg albumen, isolation of lysozyme, alcohol-insoluble cross-linked pea pod solid (AI-CLPPS), ion-exchange chromatography
Key indicators: single-crystal X-ray study; T = 295 K; mean (C-C) = 0.012 Å; disorder in main residue; R factor = 0.039; wR factor = 0.100; data-to-parameter ratio = 20.1.
CommentThe preceding study reports the structure of a tetrahedral dibromidodichloridomercurate(II), which has been isolated as the 1,2-ethanedipyridinium salt (Wang et al., 2007). Replacing the cation by 1,3-propanedipyridinium furnishes a similar tetrahalomercurate. The anion of the salt is composed 2.25 bromines and 1.75 iodines; the metal atom shows tetrahedral coordination (Fig. 1). Selected bond distances are given in Table 1.
ExperimentalThe salt was synthesized from the reaction of propane-1,3-dipyridinium dibromide (0.036 g, 0.1 mmol) in methanol (5 ml) and mercuric iodide (0.091 g, 0.2 mmol) in DMF (10 ml). The mixture was set aside for the formation of colorless crystals in 30% yield after several days.
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