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Materials produced by additive manufacturing (AM) have been extremely related to literature. However, there is still unconsolidated knowledge about the fatigue life and respective mechanisms of initiation of cracks predominant in the VHCF regime for these materials. What has been observed in materials produced by conventional routes is that fatigue cracks tend to nucleate from intrinsic defects of the material located internally or in subsurface regions. The change in the evolution process of fatigue cracks leads to the formation of a characteristic morphology on the fracture surface, known as "fish-eye". Another widespread aspect observed on the fracture surfaces is the formation of a fine granular area (FGA) nearby the initiation sites. This work aims to investigate the mechanisms of crack nucleation in VHCF of two distinct materials: conventional steel, DIN 34CrNiMo6 and AISI 316L stainless steel produced by L-DED. The ultrasonic tests were carried out at a frequency of 20±0,5 kHz and R= -1. The S-N curves were obtained and fracture surfaces were analyzed, fish-eye and FGA formation was verified. FGA sizes were compared to values estimated by empirical equations. FGA and fish-eye sizes were related to stress amplitude and maximum stress intensity factor (SIF).