We study the spectral correlation properties of the polarized fluorescence spectra of normal and cancerous human breast tissues, corresponding to patients belonging to diverse age groups and socioeconomic backgrounds. The emission range in the visible wavelength regime of 500 to 700 nm is analyzed, with the excitation wavelength at 488 nm, where flavin is one of the active fluorophores. The correlation matrices for parallel and perpendicularly polarized fluorescence spectra reveal correlated domains, differing significantly in normal and cancerous tissues. These domains can be ascribed to different fluorophores and absorbers in the tissue medium. The spectral fluctuations in the perpendicular component of the cancerous tissue clearly reveal randomization not present in the normal channel. Random matrix-based predictions for the spectral correlations match quite well with the observed behavior. The eigenvectors of the correlation matrices corresponding to large eigenvalues clearly separate out different tissue types and identify the dominant wavelengths, which are active in cancerous tissues.
Polymer blends have been obtained in the form of dimensionally stable and free standing films and their properties were characterized by different techniques. FTIR analysis and Raman spectroscopic analysis cleared the hydrogen bonding intermolecular interaction between-CONH2 groups in Poly Acrylamide (PAM) and CO -C and-CH2OH group in Poly Ethylene Oxide (PEO). From Differential Scanning Calorimeter (DSC) the study shows that crystallinity is increasing with PEO wt%. From polymer interaction parameter we also show that the polymer blend is miscible. Thermal stability of films is investigated by Thermo Gravimetric Analysis (TGA) and derivative Thermo Gravimetric Analysis (DrTG). From UV-Vis absorption spectra, absorption band edge, direct/ indirect band gap and optical activation energy have been calculated.
Using the multiresolution ability of wavelets and effectiveness of singular value decomposition (SVD) to identify statistically robust parameters, we find a number of local and global features, capturing spectral correlations in the co- and cross-polarized channels, at different scales (of human breast tissues). The copolarized component, being sensitive to intrinsic fluorescence, shows different behavior for normal, benign, and cancerous tissues, in the emission domain of known fluorophores, whereas the perpendicular component, being more prone to the diffusive effect of scattering, points out differences in the Kernel-Smoother density estimate employed to the principal components, between malignant, normal, and benign tissues. The eigenvectors, corresponding to the dominant eigenvalues of the correlation matrix in SVD, also exhibit significant differences between the three tissue types, which clearly reflects the differences in the spectral correlation behavior. Interestingly, the most significant distinguishing feature manifests in the perpendicular component, corresponding to porphyrin emission range in the cancerous tissue. The fact that perpendicular component is strongly influenced by depolarization, and porphyrin emissions in cancerous tissue has been found to be strongly depolarized, may be the possible cause of the above observation.
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