Awaad MHH, Elmenawey M and Ahmed KA (2014) Effect of a specific combination of carvacrol, cinnamaldehyde, and on the growth performance, carcass quality and gut integrity of broiler chickens, 7(4): 284-290.

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The reverse transcriptase-polymerase chain reaction/restriction fragment length polymorphism (RT-PCR/RFLP) technique was used for identification and characterization of Egyptian field strains of infectious bursal disease viruses (IBDVs) that caused severe outbreaks with (30%-60%) mortality rate. Twenty-four bursal samples collected from 24 field outbreaks in commercially reared chicken flocks experiencing signs typical of infectious bursal disease (IBD) were used. Ten of the bursal samples examined were determined to contain IBDV as evidenced by amplification of a 743-bp region of the VP2 gene of IBDV by RT-PCR. The RT-PCR products of the detected viruses were characterized by digestion with three restriction enzymes, BstN, MboI, and SpI. Three different RT-PCR/RFLP profiles were observed. Seven of the detected viruses had RFLP profiles identical to the very virulent European strains of IBDV (vvIBDVs). One virus had a RFLP profile identical to the U.S. classic vaccine strain, and one virus had a unique RFLP profile. The clinical history of the outbreaks and the presence of the SspI site in the 743-bp RT-PCR fragment were the criteria for designating the viruses as belonging to the very virulent (vv) phenotype.

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Article Info Received : 05.01.2016 Accepted : 18.04.2016 Lettuce plants were grown under sandy soil conditions in the private farm of Ahmed Orabi organization, Cairo Governorate Egypt, between 15 th November 2009 and 15 January 2010. The experiment was conducted to assess the effects of different nitrogen sources, slow release N (urea-formaldehyde) and fast release N (urea) containing fertilizers at the rates of 0, 60, 90 and 120 kg N ha -1 applied alone or combined with potassium sulphate, on lettuce plant yield. Results indicated that application of different sources of N alone or combined with potassium sulphate gave the highest fresh dry weight per plant and total lettuce yield per hectare compared with the control. The highest dry weight of lettuce was achieved with the combination of urea and potassium sulphate. Although fertilization made with the combination of urea and potassium sulphate resulted in the highest P, K, Zn and Mn contents in lettuce plant, fertilization with urea alone gave the highest N and Fe contents. The lowest content of nitrate in lettuce plants was recorded with the fertilization of urea only or with the combination of urea and potassium sulphate. Application of the combination of urea and potassium sulphate induced the highest protein content in plants. The results indicated that application of urea-formaldehyde as a slow release nitrogen fertilizer solely or combined with potassium sulphate significantly improved yield and yield quality of lettuce plants grown in sandy soil.

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