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The most conserved regions for 15 hepatitis B virus complete genome of different subtypes were aligned using PCGENE software CLUSTAL to design a new pair of primer that can bind to each subtype of hepatitis B virus (HBV), to amplify PreS region of HBV genome. A pair of primer from these conserved patches was selected using software PRIMER and named as Nhepf1 and Nhepr1. Nhepf1, forward primer bound 2362-2385 nucleotides and Nhepr1, reverse primer bound 260-283 nucleotide amplify 1.12 Kb region of HBV genome that contain PreS sequence. The pair of primer was optimized for PCR. Nhepf1 and Nhepr1 annealed well at 50°C to subtype adw2 (American), adr4 (Japanese) and Pakistanian patient derived HBV DNA without any nonspecific bands. The results were found to be highly reproducible with greater accuracy.

Badiuzzaman Said Nursi had always looked at the world as one component glorifying the one creator and testifying to His greatness. The universality he believed in, came into contradiction with the universality that results from globalization as it is generally understood and illustrated. People of economically less auspicious countries become themselves agents of globalization in their own places. This is why the effects of globalization are more harmful than those of colonialism. Globalization recruits its soldiers from among people it invades and expands to swallow the remnants of their culture and selfesteem by making them follow the model of those who are more powerful and who possess more.Badiuzzaman Said Nursi selalu memandang dunia sebagai salah satu komponen untuk memuliakan Sang Pencipta dan menyaksikan kebesaran-Nya. Universalitas yang dipercayainya, berlawanan dengan universalitas yang dihasilkan dari globalisasi seperti yang umum dipahami dan digambarkan. Masyarakat dari Negara-negara yang secara ekonomis kurang beruntung menjadikan diri mereka June 2014: 135-153 sendiri sebagai agen globalisasi di negara mereka sendiri. Inilah sebabnya mengapa efek globalisasi lebih berbahaya daripada kolonialisme. Globalisasi merekrut tentaranya dari kalangan orang-orang yang menjadi sasaran serangnya dan menelan sisa-sisa kebudayaan mereka serta harga diri dengan membuat mereka mengikuti model mereka yang lebih kuat dan memiliki kelebihan. IJIMS,Indonesian Journal of Islam and Muslim Societies, Volume 4, Number 1,

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