Various retail foods were analyzed for the presence of Listeria spp. One hundred ten samples of vegetables including lettuce, celery, tomatoes, and radishes, and 14 samples of pasteurized milk, were found to be free of L. monocytogenes. In contrast 9 of 16 (56.3%) chicken legs, 38 of 44 (86.4%) ground meats, and 6 of 30 (20%) fermented sausages contained the organism. Of 530 samples of ice cream products obtained at the manufacturing level, only 2 were positive for L. monocytogenes.
This catalogue includes all valid family-group (8 subfamilies, 52 tribes, 14 subtribes), genus-group (349 genera, 86 subgenera), and species-group names (2825 species, 215 subspecies) of darkling beetles (Coleoptera: Tenebrionidae) known to occur in North America1 and their available synonyms. Data on extant, subfossil and fossil taxa are given. For each name the author and year and page number of the description are provided, with additional information (e.g., type species for genus-group names, author of synonymies for invalid taxa) depending on the taxon rank.Several new nomenclatural acts are included. One new genus, Lepidocnemeplatia Bousquet and Bouchard, is described. Spelaebiosis Bousquet and Bouchard [for Ardoinia Özdikmen, 2004], Blapstinus marcuzzii Aalbu [for Blapstinus kulzeri Marcuzzi, 1977], and Hymenorus campbelli Bouchard [for Hymenorus oculatus Doyen and Poinar, 1994] are proposed as new replacement names. Supporting evidence is provided for the conservation of usage of Tarpela micans (Fabricius, 1798) nomen protectum over Tarpela vittata (Olivier, 1793) nomen oblitum. The generic names Psilomera Motschulsky, 1870 [= Stenomorpha Solier, 1836], Steneleodes Blaisdell, 1909 [= Xysta Eschscholtz, 1829], Ooconibius Casey, 1895 and Euconibius Casey, 1895 [= Conibius LeConte, 1851] are new synonyms (valid names in square brackets). The following 127 new synonymies of species-group names, listed in their original combination, are proposed (valid names, in their current combination, placed in square brackets): Bothrasida mucorea Wilke, 1922 [= Pelecyphorus guanajuatensis (Champion, 1884)]; Parasida zacualpanicola Wilke, 1922 [= Pelecyphorus asidoides Solier, 1836]; Stenosides kulzeri Pallister, 1954, Stenosides bisinuatus Pallister, 1954, and Parasida trisinuata Pallister, 1954 [= Pelecyphorus dispar (Champion, 1892)]; Asida favosa Champion, 1884 and Asida similata Champion, 1884 [= Pelecyphorus fallax (Champion, 1884)]; Ologlyptus bicarinatus Champion, 1884 [= Pelecyphorus indutus (Champion, 1884)]; Parasida laciniata Casey, 1912 and Parasida cristata Pallister, 1954 [= Pelecyphorus liratus (LeConte, 1854)]; Parasida esperanzae Wilke, 1922 and Parasida mixtecae Wilke, 1922 [= Pelecyphorus longipennis (Champion, 1884)]; Parasida tolucana Casey, 1912 [= Pelecyphorus scutellaris (Champion, 1884)]; Parasida purpusi Wilke, 1922 [= Pelecyphorus tristis (Champion, 1884)]; Astrotus nosodermoides Champion, 1892 [= Pelecyphorus erosus (Champion, 1892)]; Astrotus seticornis var. humeralis Champion, 1884 [= Pelecyphorus seticornis (Champion, 1884)]; Pactostoma breviuscula Casey, 1912, Pactostoma exoleta Casey, 1912, Pactostoma luteotecta Casey, 1912, Pactostoma monticola Casey, 1912, Pactostoma obtecta Casey, 1912, and Pactostoma sigillata Casey, 1912 [=Pelecyphorus anastomosis (Say, 1824)]; Ologlyptus canus Champion, 1884 and Ologlyptus sinuaticollis Champion, 1884 [= Pelecyphorus graciliformis (Solier, 1836)]; Gonasida elata reducta Casey, 1912, Gonasida elata prolixa Casey, 1912, and Gonasida aucta Casey, 1912 [...
The taxonomic concepts of Blapimorpha and Opatrinae (informal and traditional, morphology‐based groupings among darkling beetles) are tested using molecular phylogenetics and a reassessment of larval and adult morphology to address a major phylogeny‐classification gap in Tenebrionidae. Instead of a holistic approach (family‐level phylogeny), this study uses a bottom‐up strategy (tribal grouping) in order to define larger, monophyletic lineages within Tenebrioninae. Sampling included representatives of 27 tenebrionid tribes: Alleculini, Amarygmini, Amphidorini, Blaptini, Bolitophagini, Branchini, Cerenopini, Coniontini, Caenocrypticini, Dendarini, Eulabini, Helopini, Lagriini, Melanimini, Opatrini, Pedinini, Phaleriini, Physogasterini, Platynotini, Platyscelidini, Praociini, Scaurini, Scotobiini, Tenebrionini, Trachyscelini, Triboliini and Ulomini. Molecular analyses were based on DNA sequence data from four non‐overlapping gene regions: carbamoyl‐phosphate synthetase domain of rudimentary (CAD) (723 bp), wingless (wg) (438 bp) and nuclear ribosomal 28S (1101 bp) and mitochondrial ribosomal 12S (363 bp). Additionally, 15 larval and imaginal characters were scored and subjected to an ancestral state reconstruction analysis. Results revealed that Amphidorini, Blaptini, Dendarini, Pedinini, Platynotini, Platyscelidini and Opatrini form a clade which can be defined by the following morphological features: adults—antennae lacking compound/stellate sensoria; procoxal cavities externally and internally closed, intersternal membrane of abdominal ventrites 3–5 visible; paired abdominal defensive glands present, elongate, not annulated; larvae—prolegs enlarged (adapted for digging); ninth tergite lacking urogomphi. To accommodate this monophyletic grouping (281 genera and ∼4000 species), the subfamily Blaptinae sens. nov. is resurrected. Prior to these results, all of the tribes within Blaptinae were classified within the polyphyletic subfamily Tenebrioninae. The non‐monophyletic nature of Terebrioninae has already been postulated by previous authors, yet no taxonomic decisions were made to fix its status. The reinstatement of Blaptinae, which groups ∼50% of the former Tenebrioninae, helps to clarify phylogenetic relations among the whole family and is the first step towards a complete higher‐level revision of Tenebrionidae. The Central Asian tribe Dissonomini (two genera, ∼30 species) was not included in Blaptinae due to a lack of representatives in the performed phylogenetic analyses; however, based on morphological features, the tribe is listed as a potential addition to the subfamily.
Two major lineages of beetles inhabit cycad cones in the New World: weevils (Curculionoidea) in the subtribe Allocorynina, including the genera Notorhopalotria Tang and O'Brien, Parallocorynus Voss, Protocorynus O'Brien and Tang and Rhopalotria Chevrolat, and beetles in the family Erotylidae, including the genus Pharaxonotha Reitter. Analysis of the 16S ribosomal RNA (rRNA) mitochondrial gene as well as cladistic analysis of morphological characters of the weevils indicate four major radiations, with a probable origin on the cycad genus Dioon Lindl. and comparatively recent host shifts onto Zamia L. Analysis of the 16S rRNA gene for erotylid beetles indicates that an undescribed genus restricted to New World Ceratozamia Brongn. is the most early-diverging clade, and this lineage is sister to a large radiation of the genus Pharaxonotha onto Zamia, with apparent host shifts onto Dioon and Ceratozamia. Analysis of beetles are in accord with current models of continental drift in the Caribbean basin, support some proposed species groupings of cycads, but not others, and suggest that pollinator type may impact population genetic structure in their host cycads.
The microbiological quality of bottled water sold in Canada was evaluated. A total of 114 lots of bottled water, both domestic and imported, were analyzed for aerobic colony count, coliforms, fecal coliforms, and Escherichia coli. No fecal coliforms or E. coli were found. Nineteen (46%) of the 41 lots of domestic purified water were found to exceed aerobic colony count standards and another lot exceeded coliform standards. One lot each of domestic and imported mineral water exceeded coliform standards. If mineral water were governed by the aerobic colony count standards for bottled water, then five lots each of both domestic and imported mineral water would have been found to be unsatisfactory. More surveillance of the bottled water industry in Canada is recommended.
The genus Parapolycentropus, originally described for two species in 99 myo Burmese amber, is unique among Mecoptera for its long, thin proboscis and possession of just the mesothoracic pair of wings. a new series of 19 specimens with excellent preservation allows description and redescription of virtually all morphological details. Male terminalia are very similar to those of the holarctic Recent family of "snow fleas, " the Boreidae. Thoracic sclerites are highly convergent with nematocerous Diptera in the expansion of the mesothorax and great reduction of the proand metathoraces. The metathoracic wing vestige appears to be just the tegula; axial sclerites are lost. Details of the pretarsal claws are described; in P. paraburmiticus Grimaldi and Rasnitsyn the outer claw of the meso-and metathoracic pretarsi is elongate and the inner claw reduced. The proboscis is comprised not of a labial tube and "pseudolabellum" (contra Ren et al., 2009), but is mostly maxillary in origin, with the outer valves probably being galeae and the central, serrated stylet probably the hypopharynx. abdominal sternites are greatly reduced (more so in females), suggesting that the abdomen was distensible, a feature that is common in some fluid-feeding insects. The proboscis, claw, and sternite modifications indicate that Parapolycentropus fed on the hemolymph of small insects, not the blood of vertebrates.
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