WITHIN recent years a great number of determinations (which need not be mentioned in detail) have given the impression that the ferricyanide method of oxygen estimation has yielded lower results for the oxygen capacity of blood than the Van Slyke pump. This difference existed between the Van Sly k e pump and both forms of ferricyanide apparatus as currently used-Hal dane's constant pressure model and B ar croft's differential model. Such a difference has been explained: (1) onthe assumption that reducing substances in the plasma, as shown by Douglas(), Parsons and Parsons2), and also by Litarczek(3), use up oxygen during the time in which the blood is being equilibrated in the apparatus; (2) that reducing substances in the corpuscles do the same, Mo rawit z and Rohmer(4), Douglas(5); (3) that the corpuscles are incompletely laked, Haldane(6), the unlaked portions not yielding their oxygen and (4) the blood may be contaminated with organisms, Haldane(6), which have an appreciable respiration.All these explanations possess an element of truth, the first awo certainly apply to blood of anaemic individuals and probably of rabbits. Harro p (7) has drawn a general relation between the rate of oxidation in blood and the number of reticulated red cells. Nevertheless they do not cover the whole ground, because, although they are not applicable to fresh hiemoglobin solutions (as opposed to blood), such hsemoglobin solutions on occasions exhibit the phenomenon which it is sought to explain. 19-59 (3) The figure given is in each case the average of a number of determinations, the number being stated in brackets. Thus for solution I the Haldane's apparatus(s) gave 16-42, 16*42, 15.91, 16-35, 15-91, while the Van Slyke pump gave 17-15 and 16-77.
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